WGA1

GenomePlex^® Whole Genome Amplification (WGA) Kit

Kit for whole genome amplification from a variety of DNA sources including FFPE tissue

• NACRES: NA.55

Properties

• Quality Level: 200
• technique(s): whole genome amplification: suitable
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

GenomePlex^® Whole Genome Amplification WGA kit is suitable for use with genomic DNA, whole blood, buffy coats, buccal swabs, and cultured cells. Amplification requires only a small amount of starting material (1 μL blood, single cheek swab, or 10 ng of gDNA), which after PCR produces a yield of 5 - 10 μg per reaction. The amplified DNA can be purified and stored or used for downstream analyses including ABI′s TaqMan^® assays, microsatellite analysis, or sequencing. GenomePlex gives a complete representation of the entire genome with no detectable allele bias.

WGA Kit utilizes a proprietary technology based on random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable library molecules flanked by universal priming sites. WGA is achieved by PCR amplification of the library molecules using universal oligonucleotide primers.

GenomePlex method allows the researcher to generate a representative, ~500-fold amplification of genomic DNA. The amplification yield is dependent on the purity and amount of starting material. This kit contains all the required reagents to perform library preparation and fragmentation.

Application

GenomePlex^® Whole Genome Amplification (WGA) Kit has been used to amplify input and chromatin immunoprecipitation (ChIP) DNA. It has also been used in the fragmentation of bacterial artificial chromosome (BAC) clones from bacterial cultures for the preparation of repeat free (RF) probes.

Features and Benefits

• Higher yield from minimal template: Amplification of nanogram amounts (10ng) of genomic DNA to microgram yields (5-10 μg) in less than about three hours
• Nanograms of samples can be preserved at –20 °C for future use
• Choose from a variety of DNA sources: whole blood, buccal swab, blood card, plant, soil, and formalin-fixed paraffin-embedded tissue (FFPE)
• Compatible with a wide variety of downstream applications such as TaqMan^® and BeadArray assays
• Universal primers
• Whole-genome representation with no detectable bias
• Increased accuracy in amplification produces no amplicon in the negative control reactions

Other Notes

The sequences of the universal primers provided in this kit are considered proprietary.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

GenomePlex is a registered trademark of Takara Bio USA, Inc.

TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Kit Components Also Available Separately

• W4502 Water, Nuclease-Free Water, for Molecular Biology

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable