Merck
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A6964

Sigma-Aldrich

Accutase® solution

sterile-filtered, suitable for cell culture

Synonym(s):
Cell detachment solution
MDL number:
NACRES:
NA.75

sterility

sterile-filtered

Quality Level

form

liquid

concentration

400-600 units/mL

technique(s)

cell culture | mammalian: suitable

pH

6.8-7.8

shipped in

dry ice

storage temp.

−20°C

Related Categories

General description

Accutase® solution (in dulbecco′s phosphate-buffered saline (DPBS) without Ca++ or Mg++) contains proteolytic and collagenolytic enzymes for cell detachment. It is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware.

Application

Accutase® solution has been used for dissociation or digestion of nonadherent cells growing in an aggregated cluster, such as brain tumor cells, induced pluripotent stem cells (IPSCs) and glioblastoma cells into single cells.
Proven effective in detaching primary fibroblasts, endothelial cells, neurons, tumor cell lines, and insect cells. Performs exceptionally well in detaching cells for analysis of cell surface markers, virus growth assay, and flow cytometry as well as bioreactor scale-up. Does not contain mammalian or bacterial-derived products.

Features and Benefits

Ready-to-use liquid consisting of a proprietary mixture of proteolytic and collagenolytic enzymes for use in dissociation of cells from standard and adhesion-coated plasticware.

Formula variant

Prepared in Dulbecco′s PBS (0.2 g/L KCl, 0.2 g/L KH2PO4, 8 g/L NaCl, and 1.15 g/L Na2HPO4) containing 0.5 mM EDTA•4Na and 3 mg/L Phenol Red. Refer to Product Data Sheet on the website for product usage information.

Preparation Note

This product does contain phenol red. The product is shipped on dry ice and there could be significant CO2 buildup in the package. This CO2 may enter the solution and lower the pH slightly, giving an yellow-orange (around pH 6.8) vs. pinkish (around pH 7.8) color. The solution, if yellow-orange (acidic) should still be good to use as is, or sodium hydroxide may be added to adjust the pH.

Legal Information

Accutase is a registered trademark of Innovative Cell Technologies, Inc.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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