B0287

Monoclonal Anti-FITC−Biotin antibody produced in mouse

clone FL-D6, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Monoclonal Anti-FITC

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: biotin conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: FL-D6, monoclonal
• form: buffered aqueous solution
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using human tonsil
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Monoclonal Anti-FITC (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Specificity

The antibody reacts with both free and conjugated fluorescein isothiocyanate (FITC).

Immunogen

FITC-BSA conjugate

Application

Monoclonal Anti-FITC-Biotin antibody produced in mouse has been used:

• for amplification of signal in immunofluorescence assays
• in Ag-activated beads for flow-based micro immunoassay of parathyroid hormone and IL-5[1]
• enzyme linked immunosorbent assay (ELISA)

Biochem/physiol Actions

Fluorochrome labeling provides a rapid and accurate localization of the site of antigen-antibody interaction when one of the reactants from parts of a cell, tissue or other biological structure. Fluorescein isothiocyanate (FITC) is a commonly used marker for antibodies in immunofluorescence techniques since the conjugation of FITC to proteins[2] is relatively easy and does not, in general, destroy the biological activity of the labelled substances. FITC is widely used as a hapten to label different proteins and anti-FITC antibodies are used to identify these labeled proteins. Antibodies to FITC serve as universal indicator reagents by bridging FITC with another immunohistochemical reagent, such as alkaline phosphatase, horseradish peroxidase or biotin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable