Merck
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B0287

Sigma-Aldrich

Monoclonal Anti-FITC−Biotin antibody produced in mouse

clone FL-D6, purified immunoglobulin, buffered aqueous solution

Synonym(s):
Monoclonal Anti-FITC

biological source

mouse

Quality Level

conjugate

biotin conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

FL-D6, monoclonal

form

buffered aqueous solution

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using human tonsil

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

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General description

Monoclonal Anti-FITC (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Specificity

The antibody reacts with both free and conjugated fluorescein isothiocyanate (FITC).

Immunogen

FITC-BSA conjugate

Application

Monoclonal Anti-FITC-Biotin antibody produced in mouse has been used:
  • for amplification of signal in immunofluorescence assays
  • in Ag-activated beads for flow-based micro immunoassay of parathyroid hormone and IL-5[1]
  • enzyme linked immunosorbent assay (ELISA)

Biochem/physiol Actions

Fluorochrome labeling provides a rapid and accurate localization of the site of antigen-antibody interaction when one of the reactants from parts of a cell, tissue or other biological structure. Fluorescein isothiocyanate (FITC) is a commonly used marker for antibodies in immunofluorescence techniques since the conjugation of FITC to proteins[2] is relatively easy and does not, in general, destroy the biological activity of the labelled substances. FITC is widely used as a hapten to label different proteins and anti-FITC antibodies are used to identify these labeled proteins. Antibodies to FITC serve as universal indicator reagents by bridging FITC with another immunohistochemical reagent, such as alkaline phosphatase, horseradish peroxidase or biotin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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