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B352-05

Sigma-Aldrich

Bovine Pulmonary Artery Smooth Muscle Cells: BPASMC (Cryovial)

biological source

bovine pulmonary artery (tunica intima and media)

packaging

pkg of 500,000 cells

manufacturer/tradename

Cell Applications, Inc

growth mode

Adherent

karyotype

2n = 60

morphology

smooth muscle

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cardiovascular diseases

shipped in

dry ice

storage temp.

−196°C

General description

BPASMC have been used in studies relevant to pulmonary hypertension characterized by hyperproliferation of pulmonary artery smooth muscle cells, the process shown to be mediated by hypoxia-induced expression of Bcl-xl (Suzuki, 2007). Additionally, they were used to elucidate the PKC-α dependent mechanisms of proMMP-2 activation and other signaling events in smooth muscle cells under pro-inflammatory conditions related to cell growth (Chakraborti, 2009; Roy, 2011, 2013; Dey, 2012; Chowdhury, 2013; Shaikh, 2013).

Characterization: positive for smooth muscle cell specific alpha-actin expression.

Cell Line Origin

Artery

Application

cell growth, signaling, receptor transactivation and suppression, vascular remodeling

Components

Bovine Smooth Muscle Cell Basal Medium containing 10% FBS & 10% DMSO

Preparation Note

  • 2nd passage, >500,000 cells in Bovine Smooth Muscle Cell Basal Medium containing 10% FBS & 10% DMSO
  • Can be cultured at least 16 doublings

Subculture Routine

Please refer to the BPASMC Culture Protocol.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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