Merck
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B8520

Sigma-Aldrich

Anti-Mouse IgG (whole molecule)−Biotin antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

MDL number:
NACRES:
NA.46

biological source

rabbit

Quality Level

conjugate

biotin conjugate

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:200,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300
western blot: 1:400,000-1:800,000

storage temp.

−20°C

Related Categories

General description

Immunoglobulins (Igs) belongs to the immunoglobulin super-family. There are five different types of Immunoglobulins. Each immunoglobulin has two heavy (H) and two light (L) chains held together by disulfide linkages. Each light comprises of one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region.[1] IgG is abundant immunoglobulin in human serum.[2]

Immunogen

Purified mouse IgG

Application

Anti-Mouse IgG (whole molecule)-Biotin antibody has been used in
  • immunofluorescence [3]
  • immunoblot[4]
  • direct enzyme linked immunosorbent assay (ELISA)
  • serotyping assay[5]
  • immunohistochemistry[6]

Biochem/physiol Actions

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases.[7] Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.[8]

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificate of Origin

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