Merck
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C4951

Sigma-Aldrich

Cholesterol-Water Soluble

powder, BioReagent, suitable for cell culture

Synonym(s):
Cholesterol–methyl-β-cyclodextrin
NACRES:
NA.75

biological source

ovine wool

product line

BioReagent

form

powder

technique(s)

cell culture | mammalian: suitable

solubility

H2O: 300 mg/mL

shipped in

ambient

storage temp.

−20°C

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P7556E4389C3175
biological source

ovine wool

biological source

synthetic (organic)

biological source

synthetic (organic)

biological source

-

form

powder

form

powder

form

powder

form

powder

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

solubility

H2O: 300 mg/mL

solubility

H2O: 25 mg/mL, clear to slightly hazy

solubility

H2O: 25 mg/mL, clear to slightly hazy

solubility

H2O: 50-500 mg/mL (stock solution), PBS: 50-500 mg/mL (stock solution), other salt solutions: not recommended

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

-

storage temp.

−20°C

storage temp.

room temp

storage temp.

room temp

storage temp.

-

Application

Cholesterol-Water Soluble has been used:
  • in free cholesterol (FC) overloading of rat smooth muscle cells (SMCs)
  • in cytotoxicity assay
  • for the incubation of CD4+ T cells with Helicobacter pylori
  • to study the membrane cholesterol level in SH-SY5Y human neuroblastoma cells
  • to study the effects of cholesterol on cell viability and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction in primary cultured rat hippocampal neurons
  • as a supplement for growth of bacterial culture
  • to examine the interaction of S protein with angiotensin-converting enzyme 2 (ACE2)
  • to investigate the effects of cholesterol on potassium currents in the inner hair cells of guinea pigs
  • for reversible inhibition of proteinase K

Packaging

Package size based on cholesterol

Other Notes

Contains approx. 40 mg of cholesterol per gram; balance methyl-β-cyclodextrin.

Preparation Note

Preparea solution at 300mg/mL in water. Let thesolution sit undisturbed for one hour after diluting to obtain completesolubilization of product.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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