The stability of this material following multiple freeze/thaw cycles indicates that a fine precipitate may be evident. However, the performance of the material was is effected. While it is recommended to use this product within 5 days of the initial thaw, it would be up to the end user to continue use following refreezing. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/241/461/c6295pis.pdf
C6295
Cell Freezing Medium-DMSO Serum free 1x
sterile-filtered, suitable for cell culture
Synonym(s):
cryopreservation medium
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About This Item
NACRES:
NA.75
UNSPSC Code:
12352207
Pricing and availability is not currently available.
sterility
sterile-filtered
Quality Level
form
liquid
technique(s)
cell culture | mammalian: suitable, cryopreservation: suitable
impurities
endotoxin, tested
shipped in
dry ice
storage temp.
−20°C
General description
Cell freezing medium is a combination of cryoprotectant DMSO with fetal bovine serum. However serum free medium replaces serum with methylcellulose and poloxamer 188 and other additives like ascorbic acid, α-tocopherol and proline.
Application
Cell Freezing Medium-DMSO Serum free 1x has been used in the freezing of human neural progenitor cells (hNPCs), olfactory ensheathing cells (OECs) and retinal pigment epithelial (RPE) cells.
Other Notes
Complete ready-to-use medium containing Minimum Essential Medium, dimethyl sulfoxide (8.7%) and methyl cellulose. Prepared according to the formulation of Waymouth.
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This Item | |||
|---|---|---|---|
| form liquid | form liquid | form liquid | form liquid |
| sterility sterile-filtered | sterility - | sterility sterile-filtered | sterility sterile-filtered |
| technique(s) cell culture | mammalian: suitable, cryopreservation: suitable | technique(s) cell culture | mammalian: suitable, cryopreservation: suitable | technique(s) cell culture | mammalian: suitable, cryopreservation: suitable | technique(s) cell culture | mammalian: suitable |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| shipped in dry ice | shipped in wet ice | shipped in dry ice | shipped in dry ice |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Articles
Overview of good cell banking practices for cell line cryopreservation purposes.
用于细胞系冷冻保存的良好细胞库操作实践概述。
Overview on liquid nitrogen storage and freezing of cell stocks for cell line cryopreservation purposes. Free ECACC handbook download.
A novel serum-free method for culturing human prenatal retinal pigment epithelial cells
Gamm DM, et al.
Investigative Ophthalmology & Visual Science, 49(2), 788-799 (2008)
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Acute and delayed transplantation of olfactory ensheathing cells promote partial recovery after complete transection of the spinal cord
Lopez-Vales R, et al.
Neurobiology of Disease, 21(1), 57-68 (2006)
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Is it possible to thaw this product, prepare aliquots and store them at -20 degrees for further use? Thank you.
1 answer-
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