C6366

EX-CELL^® CHO Cloning Medium

Animal-component free, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Properties

• Quality Level: 400
• sterility: sterile-filtered
• form: liquid
• storage condition: protect from light
• technique(s): cell culture | mammalian: suitable (suspension)
• impurities: endotoxin, tested
• components: L-glutamine: no; NaHCO₃: yes
• shipped in: ambient
• storage temp.: 2-8°C

Description

Application

This animal component-free medium is designed to support clonal survival and growth of Chinese Hamster Ovary (CHO) cell lines, with results comparable to the traditional method using 10% fetal bovine serum.

Features and Benefits

Developed to meet the needs of the biotechnology industry, this animal component-free medium is designed for single-cell cloning of recombinant CHO cell lines adapted to serum-free suspension culture.

Other Notes

Proprietary formulation containing inorganic salts, sodium bicarbonate, essential and non-essential amino acids, vitamins, trace elements, plant hydrolysates, and other organic compounds.
Does not contain antibiotics, antimycotics, L-glutamine, insulin, or transferrin.

Caution

Do not freeze. Protect from light. Several components of the medium are light labile and should not be exposed to light for lengthy periods of time.

Reconstitution

Aseptically add 10 mL of 200 mM L-glutamine solution per 500 mL of medium prior to use.

Legal Information

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable