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C9249

Sigma-Aldrich

CryoSOfree™ DMSO-free Cryopreservation Medium

greener alternative
All Photos(1)
Synonym(s):
cell freezing medium
NACRES:
NA.75

form

liquid

Quality Level

200

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

technique(s)

cell culture | mammalian: suitable
cryopreservation: suitable

greener alternative category

Aligned

shipped in

wet ice

storage temp.

2-8°C

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This Item
C6295C2999C2874
CryoSOfree™ DMSO-free Cryopreservation Medium

Sigma-Aldrich

C9249

CryoSOfree™ DMSO-free Cryopreservation Medium

Sigma-Aldrich

Sigma-Aldrich

C6295

Cell Freezing Medium-DMSO Serum free 1x

CryoStor® cell cryopreservation media CS5

Sigma-Aldrich

C2999

CryoStor® cell cryopreservation media

CryoStor® cell cryopreservation media CS10

Sigma-Aldrich

C2874

CryoStor® cell cryopreservation media

technique(s)

cell culture | mammalian: suitable, cryopreservation: suitable

technique(s)

cell culture | mammalian: suitable, cryopreservation: suitable

technique(s)

cell culture | mammalian: suitable, cryopreservation: suitable

technique(s)

cell culture | mammalian: suitable, cryopreservation: suitable

shipped in

wet ice

shipped in

dry ice

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

-

Quality Level

100

Quality Level

100

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

-

greener alternative product characteristics

-

greener alternative product characteristics

-

General description

Cultured cells in a suspension can be cryopreserved with minimal damage from freezing and thawing steps. CryoSOfree™ DMSO-free cryopreservation medium preserves them with high viability and physiological activity. This medium is recommended for the preservation of stem cells, hepatocytes, tissue sample and a wide variety of cell types and established cell lines. CryoSOfree™ DMSO-free cryopreservation medium is a proprietary formulation, containing no DMSO nor animal proteins. It is ready-to-use and complete with no additives required.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of DMSO for cryopreservation of cell cultures. For more information see here.

Application

CryoSOfree is a chemically defined DMSO-free cryopreservation medium. It is animal component free and has been shown to preserve the potential of stem cell for differentiation.

Features and Benefits

  • Preserves pluripotency of stem cells
  • Low cytotoxicity
  • Chemically defined, serum free, protein free
  • Ready to use


The following cell lines have been recovered at ≥90% following cryopreservation with CryoSOfree:
L929, KB, MC3T3, B16F1, Colon26, CaCO2, HT1080, MG63

Preparation Note

1 ml of CyroSOfree is sufficient for 5x105- 5x106 cells.

Legal Information

CryoSOfree is a trademark of Sigma-Aldrich Co. LLC

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290 - H315 - H319

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Kazuaki Matsumura et al.
Cell transplantation, 19(6), 691-699 (2010-06-08)
Cryoprotective agents (CPAs) such as dimethyl sulfoxide (DMSO), glycerol, ethylene glycol, and propylene glycol have been used for the cryopreservation of cells and tissues. DMSO is the most effective CPA but shows high cytotoxicity and can effect differentiation. ɛ-poly-L-lysine (PLL)
Kazuaki Matsumura et al.
Biomaterials, 30(27), 4842-4849 (2009-06-12)
Dimethyl sulfoxide (DMSO) has been used for several decades as the most efficient cryoprotective agent (CPA) for many types of cells and tissues in spite of its cytotoxicity and its effects on differentiation. Here we report that polyampholytes with an
Matthew A Schaller et al.
JCI insight, 6(18) (2021-08-07)
Cell lines are the mainstay in understanding the biology of COVID-19 infection but do not recapitulate many of the complexities of human infection. The use of human lung tissue is one solution for the study of such novel respiratory pathogens.

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