CAS9BST

CAS9 Blasticidin Lenti Plasmid

Name: CAS9 Blasticidin Lenti Plasmid
Brand: SIGMA

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About This Item

UNSPSC Code:

41106610

NACRES:

NA.51

Key Documents

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recombinant

expressed in E. coli

Quality Level

200

packaging

vial of 50 μL

concentration

20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)

selection

ampicillin
blasticidin

shipped in

dry ice

storage temp.

−20°C

Related Categories

CRISPR Cas9 Proteins & Gene Editing

Gene Editing & Functional Genomics

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This Item	LVCAS9NEO	DCAS9P300VP	CAS9HYGROV
Sigma-Aldrich CAS9BST CAS9 Blasticidin Lenti Plasmid	Sigma-Aldrich LVCAS9NEO Cas9 Neomycin Lenti Particles	Sigma-Aldrich DCAS9P300VP Sigma CRISPR dCAS9-p300V Activator Lenti Plasmid	Sigma-Aldrich CAS9HYGROV Cas9 Hygromycin Lentiviral Particles
recombinant expressed in E. coli	recombinant -	recombinant expressed in E. coli	recombinant -
storage temp. −20°C	storage temp. −70°C	storage temp. −20°C	storage temp. −70°C
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in dry ice
packaging vial of 50 μL	packaging vial of 200 μL	packaging vial of 50 μL	packaging vial of 200 μL
concentration 20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)	concentration ≥1 x 10 ⁶ VP/ml virus (via p24 assay)	concentration 20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)	concentration ≥1 x 10 ^{6^{VP/ml virus (via p24 assay)}}
selection ampicillin	selection neomycin / G418	selection -	selection blasticidin

General description

This product is a blasticidin resistant lenti-Cas9 plasmid for generation of lentiviral particles. Use Sigma′s lentiviral Cas9 reagents to efficiently generate stable cell lines expressing Cas9 for CRISPR genome editing. Sigma′s lenti-Cas9 plasmid is one part of a two-vector CRISPR system with individual Cas9 and gRNA expression vectors. Please see the link below to order gRNA.

Application

Functional Genomics/Screening /Target Validation/Genome Editing

Other Notes

Click here to order gRNA plasmid and virus.

1ug of plasmid DNA, EF1a-Cas9-2A-Blasticidin Lenti

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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