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MCF7 Cell Line human

86012803, from human breast(adenocarcinoma)

Synonym(s):
IBMF-7 Cells, Michigan Cancer Foundation-7 Cells, ssMCF-7 Cells, MCF7 Cells
eCl@ss:
32190102

biological source

human breast (adenocarcinoma)

growth mode

Adherent

karyotype

2n = 46, hypertriploid to hypotetraploid

morphology

Epithelial-like

products

Not specified

receptors

Reported to express the oestrogen and progesterone receptor

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human Caucasian breast adenocarcinoma

Cell Line Description

Established from the pleural effusion from a 69 year female caucasian suffering from a breast adenocarcinoma. Cells exhibit some features of differentiated mammary epithelium including oestradiol synthesis and formation of domes. Cells may carry B or C type retrovirus and are considered to represent a category 2 pathogen (P2 containment). Cells express both the wild type and variant oestrogen receptors as well as progesterone receptor. The cells should be handled under laboratory containment level 2.

Application

Tumourigenicity and virus studies: Type B and C viruses

DNA Profile

STR-PCR Data:
Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 8,9
THO1: 6
TPOX: 9,12
vWA: 14,15

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids NEAA + 10% Foetal Bovine Serum FBS / FCS.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% Trypsin or Trypsin/EDTA; 5% CO2; 37°C.

Other Notes

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Certificate of Analysis

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Certificate of Origin

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