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D0818

Sigma-Aldrich

Decalcifying Solution-Lite

bp 208 °F, aqueous solution

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Synonym(s):
universal decalcifying agent
UNSPSC Code:
12171500
NACRES:
NA.47

form

aqueous solution

Quality Level

shelf life

5 yr

color

light yellow

bp

208 °F

solubility

water: soluble

application(s)

hematology
histology

storage temp.

room temp

General description

Decalcifying Solution-Lite is designed to be a universal and effective decalcifying agent.

Application

Decalcifying Solution-Lite is intended to be used for the decalcification of routine, immunohistochemical, and bone marrow core specimens.
It works equally well with all types of samples, including calvarial bone samples in an easy-to-handle time frame.

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A Merchán-Pérez et al.
Histochemistry and cell biology, 112(2), 125-130 (1999-08-25)
An ascorbic acid decalcifying solution was applied to immuno- and affinohistochemical studies on the inner ear. Rat inner ears fixed in 4% paraformaldehyde in PBS or in 2% acetic acid in ethanol solutions were adequately decalcified in an ascorbic acid
Farhana Begum et al.
Journal of neuroscience methods, 187(1), 59-66 (2010-01-02)
Histological analysis of bone encased tissue is severely hampered by technical difficulties associated with sectioning calcified tissue. Cryosectioning of bone is possible but requires significant technical adaptation and expensive materials and is often time-consuming. Some decalcifying reagents in common use
P J Emans et al.
Biotechnic & histochemistry : official publication of the Biological Stain Commission, 80(3-4), 111-115 (2005-11-22)
Apoptosis is characterized by DNA strand breaks with a 3'-OH terminus, which are analyzed by terminal deoxy(d)-UTP nick end labeling (TUNEL). Proteinase K digestion is thought to be an essential step in the TUNEL procedure. The effects of decalcifying reagents
L F Machado-Silveiro et al.
International endodontic journal, 37(6), 365-369 (2004-06-10)
To measure the demineralization capability of 1 and 10% citric acid, 10% sodium citrate and 17% EDTA during immersions of 5, 10 and 15 min on root canal dentine. Crowns were sectioned from eight maxillary canines. The cementum was removed
Juliana Dos Santos Neves et al.
Acta histochemica, 113(4), 484-488 (2010-07-06)
This study was designed to verify the influence of three demineralizing agents on EGF and EGFR immunostaining as well as on tissue morphology. We chose submandibular glands that are a source of EGF and its receptor and which could be

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