The volume of the container is 2 mL. Reconstitution of this product should be performed using 0.2 µm filtered 20 mM Tris, pH 7.0, to prepare a stock solution of >= 25 ug/mL. This stock solution may be diluted immediately before use to achieve the final working concentration. Additional filtration is not recommended, as it may result in product loss due to adsorption onto the filter membrane.
F0291
Fibroblast Growth Factor-Basic, human
≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym(s):
Fibroblast Growth Factor-Basic human, FGF2, bFGF, hBFGF
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Pack Size | SKU | Availability | Price |
|---|
About This Item
Biological source:
human
Recombinant:
expressed in E. coli
Assay:
≥97% (SDS-PAGE)
Form:
lyophilized powder
Mol wt:
16.0 kDa
Impurities:
≤1.00 EU/μg
Pricing and availability is not currently available.
Product Name
hBFGF, FGF-Basic, recombinant, expressed in E. coli, suitable for cell culture
biological source
human
Quality Level
recombinant
expressed in E. coli
assay
≥97% (SDS-PAGE)
form
lyophilized powder
potency
≤-0.6 ng/mL
mol wt
16.0 kDa
packaging
pkg of 4X25 μg, pkg of 25 μg
storage condition
avoid repeated freeze/thaw cycles
1 of 1
This Item | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) cell culture | stem cell: suitable | technique(s) cell culture | mammalian: suitable |
| assay ≥97% (SDS-PAGE) | assay ≥97% (SDS-PAGE) | assay >95% (SDS-PAGE) | assay >95% (SDS-PAGE) |
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant - | recombinant expressed in HEK 293 cells |
| biological source human | biological source human | biological source human | biological source human |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
| form lyophilized powder | form lyophilized powder | form - | form lyophilized powder |
General description
Fibroblast growth factor-basic (FGF-Basic) is found in basement membranes and sub-endothelial extracellular matrix. FGF-Basic is induced early in the development of the embryo where it is necessary (but not sufficient) for pluripotency and self-renewal of embryonic stem cells (ESC) and lineage defined stem cells; wherein it functions to support proliferation and inhibit differentiation. Self-renewal of hESC depends upon activation of the Activin/Nodal/Smad2,3 branch and suppression of the BMP/GDF/Smad5 branch of the TGFβ-family cell signaling program. FGF-Basic is a competence factor for Nodal support of pluripotency. Activin-A is necessary and sufficient to maintain stemness. It induces the expression of both Nodal and FGF-Basic in human ES cells.
Application
Physical form
Lyophilized from 0.2 μm filtered solution in 20 mM Tris and 1 M NaCl, pH 7.0, containing 50 μg bovine serum albumin per 1 μg BFGF.
Analysis Note
The bioactivity of FGF-Basic was measured in a fluorometric assay using the redox sensitive dye, Resazurin.
related product
Product No.
Description
Pricing
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
R Beklem Bostancioglu et al.
Colloids and surfaces. B, Biointerfaces, 155, 415-428 (2017-05-02)
Accelerated Mesenchymal Stem Cells (MSCs) condensation and robust MSC-matrix and MSC-MSC interactions on nano-surfaces may provide critical factors contributing to such events, likely through the orchestrated signal cascades and cellular events modulated by the extracellular matrix. In this study, human
C Xu et al.
Nature biotechnology, 19(10), 971-974 (2001-10-03)
Previous studies have shown that maintenance of undifferentiated human embryonic stem (hES) cells requires culture on mouse embryonic fibroblast (MEF) feeders. Here we demonstrate a successful feeder-free hES culture system in which undifferentiated cells can be maintained for at least
Lei Xiao et al.
Stem cells (Dayton, Ohio), 24(6), 1476-1486 (2006-02-04)
Human embryonic stem cells (hESCs) self-renew indefinitely while maintaining pluripotency. The molecular mechanism underlying hESCs self-renewal and pluripotency is poorly understood. To identify the signaling pathway molecules that maintain the proliferation of hESCs, we performed a microarray analysis comparing an
Chunhui Xu et al.
Stem cells (Dayton, Ohio), 23(3), 315-323 (2005-03-08)
Previous studies have shown that prolonged propagation of undifferentiated human embryonic stem cells (hESCs) requires conditioned medium from mouse embryonic feeders (MEF-CM) as well as matrix components. Because hESCs express growth factor receptors, including those for basic fibroblast growth factor
Structural characterization and biological functions of fibroblast growth factor.
D Gospodarowicz et al.
Endocrine reviews, 8(2), 95-114 (1987-05-01)
Articles
Role of growth factors in stem cell differentiation and various growth factors for your research at sigmaaldrich.com
新型无血清、无异种人间充质干细胞（MSC）骨细胞分化培养基的开发。
用于细胞培养的成纤维细胞生长因子，以及用于您的研究的各类生长因子
Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.
Protocols
A stem cell culture protocol to generate 3D NSC models of Alzheimer’s disease using ReNcell human neural stem cell lines.
有关间充质的信息，特别是间充质干细胞培养实验方案用于间充质干细胞（MSC）分离、扩增和分化的分步细胞培养方案。
Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.
Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.
-
What is the volume of the vial and how do I reconstitute the powder?
1 answer-
Helpful?
-
-
Hey! I am using this product to prepare a stem cell media. I know the concentration is 0.025mg but it has two data sheets, one that its soluble in water (0.250ml) and other that says to dissolve in 20mM Tris Base pH 7.0. How many volume should I add?
1 answer-
The solubility of this product is tested in water. It is lyophilized from a solution containing 20 mM Tris and 1M NaCl with 50 ug BSA as a stabilizer. The product data sheet recommends reconstituting in the 20 mM Tris for optimum stability and long term storage.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service