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G5893

Sigma-Aldrich

Gamborg′s B-5 Basal Medium with Minimal Organics

fine powder, suitable for plant cell culture

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NACRES:
NA.72

form

fine powder

Quality Level

technique(s)

cell culture | plant: suitable

application(s)

agriculture

storage temp.

2-8°C

General description

Gamborg′s B-5 basal medium is an efficient nutrient mixture for plants.
Gamborg′ B-5 medium may be purchased as a complete medium (G5893) or reconstituted from Vitamin 1000x (G1019) and Basal Salt Mixture (G5768) chemstocks. Use Gamborg′s B-5 (B5) medium for in vitro culture of plant cell lines and tissues such as of Taxus globosa, Splachnum ampullaceum Hedw, Linum album, Eucalyptus camaldulensis. Gamborg′s B-5 medium has been used for the culture of Annona squamosa callus.

Application

Gamborg′s B-5 Basal Medium with Minimal Organics has also been used:
  • as a component of liquid media for the tissue culture of Brassica rapa
  • as a component of callus inducing medium for culturing Arabidopsis cardiolipin mutant seedlings
  • as a component of solid GM medium for germination of Arabidopsis seeds

Formula variant

With the macro- and micronutrients, and vitamins as described by Gamborg, et al. (1968).

Media Formulation

Quantity

Formulated to contain 3.2 grams of powder per liter of medium.

Pictograms

Flame over circle

Signal Word

Warning

Hazard Statements

Hazard Classifications

Ox. Sol. 3

Storage Class Code

5.1B - Oxidizing hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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