G7765

Gelatin from cold water fish skin

40-50% in H₂O

• Synonym(s): Gelatine, Teleostean gelatin
• CAS Number: 9000-70-8
• MDL number: MFCD00081638
• NACRES: NA.61

Properties

• biological source: fish skin
• Quality Level: 200
• form: liquid
• mol wt: ~60 kDa
• contains: 0.15% propyl p-hydroxybenzoate as preservative; 0.2% methyl p-hydroxybenzoate as preservative
• concentration: 40-50% in H₂O
• technique(s): ELISA: suitable; cell culture | mammalian: suitable; immunocytochemistry: suitable; western blot: suitable
• storage temp.: 2-8°C

Description

General description

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, bones, etc. in water.

Gelatin is derived from collagen and is the most abundant protein in the extracellular matrix (ECM) of the tissues. It is a bioactive material. Gelatin consists of matrix metalloproteinase (MMP) sensitive protein sequences.

Application

Gelatin from cold water fish skin has been used in an incubation medium (IM) in immunolabelling of rat brain sections, as a component of rinse buffer in western blotting of various cells, as a blocking agent in immunofluorescent staining of HeLa cells.

Used as a blocking agent in immunochemistry.

Biochem/physiol Actions

Gelatin is cross-linked either chemically or enzymatically to form a three dimensional (3D) hydrogel.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves