Merck
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GAHK20

Supelco

Glucose (HK) Assay Kit

sufficient for 20 assays

NACRES:
NA.84

usage

sufficient for 20 assays

Quality Level

analyte chemical class(es)

sugars (glucose)

technique(s)

photometry: suitable

application(s)

food and beverages
general analytical

storage temp.

2-8°C

Application

This kit is for the quantitative, enzymatic determination of glucose in food and other material. Glucose is phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Glucose-6-phosphate (G6P) is then oxidized to 6-phospho-gluconate in the presence of oxidized nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to glucose concentration.

Kit Components Only

Product No.
Description

  • glucose (HK) assay reagent 20 mL

  • glucose standard .5 mL

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

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