H2387

Hanks′ Balanced Salts

Modified, without calcium chloride, magnesium sulfate and sodium bicarbonate, powder, suitable for cell culture

• Synonym(s): HBSS
• MDL number: MFCD00217418
• NACRES: NA.75

Properties

• form: powder
• technique(s): cell culture | mammalian: suitable
• storage temp.: 2-8°C

Description

General description

Balanced salt solution (BSS) provides cells with water, bulk inorganic ions, and buffering system to maintain the normal cell metabolism and physiological range (pH 7.2-7.6), respectively. It also acts as an irrigating, diluting, and transporting agent and maintains the intracellular and extracellular osmotic balance. BSS also provides energy sources such as glucose for cell metabolism.

Application

Hanks′ Balanced Salt has been used:

• to culture primary hippocampal neuron cells from rats
• to collect the rat brains
• to culture rat primary hepatocytes

Packaging

1, 1, 2000 L

1 pkg

Quantity

Formulated to contain 9.5 grams of powder per liter of medium.

Reconstitution

Supplement with 0.35 g/L sodium bicarbonate.

Other Notes

The pH is adjusted after addition of sodium bicarbonate.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves