H3375

HEPES

≥99.5% (titration)

• Synonym(s): 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
• Empirical Formula (Hill Notation): C₈H₁₈N₂O₄S
• CAS Number: 7365-45-9
• Molecular Weight: 238.30
• Beilstein: 883043
• EC Number: 230-907-9
• MDL number: MFCD00006158
• PubChem Substance ID: 24895572
• NACRES: NA.25

Properties

• Quality Level: 400
• Assay: ≥99.5% (titration)
• form: crystalline powder
• technique(s): cell culture | mammalian: suitable
• pH: 5.0-6.5 (25 °C, 238 g/L)
• useful pH range: 6.8-8.2
• pKa (25 °C): 7.5
• solubility: water: 500 mg/mL, clear, colorless
• application(s): diagnostic assay manufacturing
• SMILES string: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Description

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

HEPES has been used:

• As a component in Danieau medium, which is used to prevent melanin pigment formation post gastrulation of zebra-fish embryo
• As a component of Hanks′ solution used in the degradation testing of Iron
• As a component of HEPES medium in the transfection of cell culture with plasmids
• Along with RPMI-1640, L-glutamine, FBS, Sodium pyruvate and β-mercaptoethanol for the culturing of rat insulinoma cells
• As a supplement in TCM199 medium for washing fresh oocytes required for nuclear transfer studies

Other Notes

Go to Redi-Dri^™ Product Listing RDD002
Easily compare specifications for HEPES products with the HEPES specification table.

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves