Merck
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H4641

Sigma-Aldrich

Hanks′ Balanced Salt Solution 10x

Without calcium chloride, magnesium sulfate and sodium bicarbonate, 10 ×, liquid, sterile-filtered, suitable for cell culture

Synonym(s):
Hanks’ Balanced Salt solution, HBSS
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

form

liquid

concentration

10 ×

technique(s)

cell culture | mammalian: suitable

components

phenol red: yes

shipped in

ambient

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General description

Balanced salt solution (BSS) is crucial in cell culture as it serves as an irrigating, transporting, and diluting agent to maintain the intra- and extracellular osmotic balance and also provides energy for cell metabolism. It provides water and bulk inorganic ions to the cells to maintain the normal cell metabolism. BSS plays a role in the buffering system to maintain the medium within the physiological pH range (7.2-7.6).

Application

Hanks′ Balanced Salt Solution 10x has been used:

  • as a diluting agent during the Chinese hamster ovary cell line detachment from growth medium[1]
  • to wash the biopsy specimens of lamina propria lymphocytes (LPLs)[2]
  • as a wash solution to wash the fish cell line during removal from growth medium[3]

Reconstitution

Supplement with 0.35 g/L sodium bicarbonate at 1X.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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