I2911

StableCell^™ IMDM

With stable glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): Iscove’s Modified Dulbecco’s Medium, IMDM
• MDL number: MFCD00217469

Properties

• Quality Level: 400
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | hybridoma: suitable; cell culture | mammalian: suitable
• impurities: Endotoxin, tested
• components: NaHCO₃: yes; glucose: yes; stable glutamine: yes; phenol red: yes
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

This IMDM differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.

Application

Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove′s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Legal Information

StableCell is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable