Merck
All Photos(1)

Documents

L3522

Sigma-Aldrich

LB Broth (Miller)

Highly-referenced nutrient-rich microbial growth powder medium, suitable for regular E.coli culture

Sign Into View Organizational & Contract Pricing

Synonym(s):
Luria Broth, Miller’s LB Broth
NACRES:
NA.85

grade

for molecular biology

Quality Level

description

Miller′s Modification, 10 g/L NaCl

sterility

non-sterile

form

powder

composition

NaCl, 10 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L

pH

6.8-7.2(2.5% solution)

application(s)

food and beverages

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
L2542L3147L3272
vibrant-m

L3522

LB Broth (Miller)

vibrant-m

L2542

LB Broth (Miller)

vibrant-m

L3147

LB Broth with agar (Miller)

vibrant-m

L3272

LB Broth with agar (Luria low salt)

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

form

powder

form

liquid

form

powder

form

powder

sterility

non-sterile

sterility

sterile; 0.2 μm filtered

sterility

non-sterile

sterility

non-sterile

application(s)

food and beverages

application(s)

microbiology

application(s)

food and beverages
microbiology

application(s)

food and beverages
microbiology

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

General description

Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates.

Application

LB Broth (Miller) has been used as a culture medium for E coli, Pseudomonas putida KT2440, Vibrio parahaemolyticus, and soil samples for the isolation of Bacillus spp.
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.

Biochem/physiol Actions

Luria Broth is used for maintenance and propagation of Escherichia coli. The E. coli grow faster in Luria Broth because the tryptone and yeast supply essential growth factors that the E. coli would otherwise have to synthesize. Luria Broth also contains essential electrolytes for transport and osmotic balance, due to the NaCl component.

Features and Benefits

Miller′s LB powder provides:
  • Easy scale-up using larger package sizes
  • A budget-friendly alternative to liquid
  • Standard formulation

Preparation Note

1. Suspend 25 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121 °C.
To prepare the medium of Luria, Adams and Ting (also known as LC broth: Aseptically add 25 ml of sterile 0.1 M calcium chloride after autoclaving.

Reconstitution

Stir to suspend 25g powder in 1L water. Autoclave for 15 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 7

1 of 7

Ikhlaas M Kasli et al.
AMB Express, 9(1), 5-5 (2019-01-09)
Production of recombinant proteins such as antibody fragments in the periplasm of the bacterium Escherichia coli has a number of advantages, including the ability to form disulphide bonds, aiding correct folding, and the relative ease of release and subsequent capture
Demeng Tan et al.
Microorganisms, 8(3) (2020-03-12)
Phage therapy is a potential and promising avenue for controlling the emergence and spread of multidrug-resistant (MDR) Klebsiella pneumoniae, however, the rapid development of anti-phage resistance has been identified as an obstacle to the development of phage therapy. Little is
Single-cell bacteria growth monitoring by automated DEP-facilitated image analysis
Peitz I and van Leeuwen R
Lab on a chip, 10(21), 2944-2951 (2010)
Selecting lipopeptide-producing, Fusarium-suppressing Bacillus spp.: Metabolomic and genomic probing of Bacillus velezensis NWUMFkBS10. 5
Adeniji A, et al.
MicrobiologyOpen, e742-e742 (2018)
Transcriptional profiling of Vibrio parahaemolyticus exsA reveals a complex activation network for type III secretion
Liu AC and Thomas NA
Frontiers in Microbiology, 6, 1089-1089 (2015)

Articles

The development of genetic engineering and cloning has opened many possibilities of expression and isolation of heterologous proteins for research purposes. Considerable advances in technology have enabled expression and isolation of recombinant proteins in large scale.

Whole transcriptome amplification (WTA) is a method often used to amplify small quantities of reverse transcribed RNA, or degraded RNA for direct use in Next-Generation Sequencing (NGS) workflows. Discover our SeqPlex™-I WTA library preparation kit for producing libraries that are ready for direct input onto Illumina® NGS flow cells.

Protocols

General protocols for growth of competent cells in microbial medium.

Related Content

KOD One™ PCR Master Mix overview for ultra-fast PCR with high specificity, fidelity, and yield

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service