L3522

LB Broth (Miller)

Highly-referenced nutrient-rich microbial growth powder medium, suitable for regular E.coli culture

• Synonym(s): Luria Broth, Miller’s LB Broth
• NACRES: NA.85

Properties

• grade: for molecular biology
• Quality Level: 200
• description: Miller′s Modification, 10 g/L NaCl
• sterility: non-sterile
• form: powder
• composition: NaCl, 10 g/L ; Tryptone, 10 g/L ; Yeast Extract, 5 g/L
• pH: 6.8-7.2(2.5% solution)
• application(s): food and beverages
• storage temp.: room temp
• suitability: nonselective for Escherichia coli; nonselective for coliforms

Description

General description

Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates.

Application

LB Broth (Miller) has been used as a culture medium for E coli, Pseudomonas putida KT2440, Vibrio parahaemolyticus, and soil samples for the isolation of Bacillus spp.
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.

Biochem/physiol Actions

Luria Broth is used for maintenance and propagation of Escherichia coli. The E. coli grow faster in Luria Broth because the tryptone and yeast supply essential growth factors that the E. coli would otherwise have to synthesize. Luria Broth also contains essential electrolytes for transport and osmotic balance, due to the NaCl component.

Features and Benefits

Miller′s LB powder provides:

• Easy scale-up using larger package sizes
• A budget-friendly alternative to liquid
• Standard formulation

Preparation Note

1. Suspend 25 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121 °C.
To prepare the medium of Luria, Adams and Ting (also known as LC broth: Aseptically add 25 ml of sterile 0.1 M calcium chloride after autoclaving.

Reconstitution

Stir to suspend 25g powder in 1L water. Autoclave for 15 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable