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L7025

Sigma-Aldrich

LB Broth with agar (Lennox)

Tablet microbial growth medium

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Synonym(s):
LB Agar, LB agar
NACRES:
NA.85

grade

for molecular biology

Quality Level

200

sterility

non-sterile

form

tablet

application(s)

food and beverages

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

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L7533L7658L3022
LB Broth with agar (Lennox) Tablet microbial growth medium

Sigma-Aldrich

L7025

LB Broth with agar (Lennox)

LB Broth with agar (Lennox) EZMix™ powder microbial growth medium

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L7533

LB Broth with agar (Lennox)

LB Broth (Lennox) EZMix™ powder microbial growth medium

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LB Broth (Lennox)

LB Broth (Lennox) Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture.

Sigma-Aldrich

L3022

LB Broth (Lennox)

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

form

tablet

form

powder

form

powder

form

powder

application(s)

food and beverages

application(s)

agriculture
food and beverages

application(s)

agriculture
food and beverages

application(s)

food and beverages

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

General description

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

Application

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.

Features and Benefits

Lennox LB tablet with agar provides:
  • A budget-friendly alternative to pre-poured plates
  • Convenient small package to eliminate weighing
  • Fast preparation – no stirring required

Components

13.72g/L Agar
9.14g/L Tryptone
4.57 g/L Yeast Extract
4.57 g/L NaCl
1.6g/L inert binding agents

Reconstitution

Add each 1.68 g tablet to 48.3 mL water (no stirring required). Autoclave for 20 minutes at 121 °C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired). Pour into petri dishes and allow to solidify.

Legal Information

Zeocin is a registered trademark of Cayla Sarl

related product

Product No.
Description
Pricing

L7275

LB Broth (Lennox), Tablet microbial growth medium

L7658

LB Broth (Lennox), EZMix™ powder microbial growth medium

L7533

LB Broth with agar (Lennox), EZMix™ powder microbial growth medium

L3022

LB Broth (Lennox), Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture.

L2897

LB Broth with agar (Lennox), Highly-referenced microbial growth powder medium with Agar, low salt, suitable for salt-sensitive E. coli culture.

L5542

LB Agar Plates, pre-poured LB agar plates for E. coli

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SIG10 Chemically Competent Cells, for protein expression and DNA plasmid production

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SIG10 5a Chemically Competent Cells, for DNA plasmid production

CMC0014

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Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Atlas, R. M., L.C. Parks
Handbook of Microbiological Media (1993)
Shun Gen Huang et al.
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Protocols

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

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