M0268

Minimum Essential Medium Eagle

With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

• Synonym(s): EMEM, MEM
• NACRES: NA.75

Properties

• Quality Level: 500
• form: powder
• technique(s): cell culture | mammalian: suitable
• components: HEPES: no; phenol red: yes; Earle’s salts (5% CO₂): yes; NaHCO₃: no; L-glutamine: yes; sodium pyruvate: no
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Minimum Essential Medium Eagle (MEM) is one of the most widely used synthetic cell culture media. MEM has been used for culturing several cells grown in monolayers. It is commonly used to grow attached cell lines, such as fibroblasts, in the presence of fetal bovine sera (FBS), calf, or horse sera.

Application

Minimum Essential Medium Eagle has been used:

• in the isolation and culture of primary hepatocytes
• to culture the human endometrial carcinoma cells Ishikawa (ATTC) and L-Wnt-3a cells (ATCC) that secrete Wnt family member 3a (Wnt-3a) ligand
• to culture the AN3CA (endometrial cancer cell line) cells, and HEC1-B human (endometrial adenocarcinoma cell line) cells

Features and Benefits

• Minimum Essential Medium Eagle (MEM) contains higher concentrations of amino acids to mimic the protein composition of cultured mammalian cells.
• The formulation has been altered by optional elimination of calcium to enable the growth of cells in suspension culture.

Quantity

Formulated to contain 9.5 grams of powder per liter of medium.

Reconstitution

Supplement with 2.2 g/L sodium bicarbonate.

Other Notes

Essentially the same as the original formula, except it is provided as a powder.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable