Merck
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M2645

SAFC

Minimum Essential Medium Eagle

HEPES Modification, with Earle′s salts, L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):
MEM
NACRES:
NA.75

form

powder

technique(s)

cell culture | mammalian: suitable

components

HEPES: 25 mM
phenol red: yes
L-glutamine: yes
NaHCO3: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes

shipped in

ambient

storage temp.

2-8°C

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This Item
FG0325M0643M0644
SAFC

SAFC

M2645

Minimum Essential Medium Eagle

SAFC

SAFC

FG0325

Minimum Essential Medium Eagle

form

powder

form

liquid

form

powder

form

powder

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

HEPES: 25 mM
phenol red: yes
L-glutamine: yes
NaHCO3: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes

components

stable glutamine: yes
L-glutamine: no
phenol red: yes
sodium pyruvate: no
Earle’s salts (5% CO2): yes
HEPES: no
Hanks’ salts (2% CO2): no
glucose: yes
NaHCO3: yes

components

HEPES: no
Earle’s salts (5% CO2): yes
NaHCO3: no
phenol red: yes
L-glutamine: yes
sodium pyruvate: no

components

NaHCO3: no
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

classic media, classical media, FBS

-

classic media, classical media, FBS

classic media, classical media, FBS, hybrid cells

Quantity

Formulated to contain 14.2 grams of powder per liter of medium.

Reconstitution

Supplement with 2.2 g/L sodium bicarbonate.

Other Notes

Leaving the bicarbonate out gives the investigator greater control over the buffer system.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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