M3024

Minimum Essential Medium Eagle

with Earle′s salts and non-essential amino acids, without L-glutamine, phenol red and sodium bicarbonate, Modified, powder, suitable for cell culture

• Synonym(s): EMEM, MEM
• NACRES: NA.75

Properties

• Quality Level: 500
• form: powder
• technique(s): cell culture | mammalian: suitable
• components: Earle’s salts (5% CO₂): yes; L-glutamine: no; HEPES: no; NaHCO₃: no; sodium pyruvate: no; phenol red: no
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Minimum Essential Medium Eagle contains 19 amino acids. The essential amino acids and the non-essential amino acids: L-ala, L-asn, L-asp, L-glu, L-gly, L-pro, and L-ser. This medium is preferred for the growth of adherent cells in the presence of reduced serum or as a base for the development of a serum-free medium. It is suitable for a wide variety of cells grown in monolayers.

Application

Minimum Essential Medium Eagle has been used:

• to culture endometrial adenocarcinoma Ishikawa cells for bisphenol studies
• to culture MCF-7 cells for experimental treatments with hormone
• to maintain zebrafish pineal glands for action spectrum analysis

Features and Benefits

Contains high concentrations of amino acids so the medium resembles the protein composition of cultured mammalian cells

Quantity

Formulated to contain 9.3 grams of powder per liter of medium.

Reconstitution

Supplement with 0.292 g/L L-glutamine, 2.2 g/L sodium bicarbonate.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable