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Merck

M3900

Murashige and Skoog Vitamin Solution

1000 ×, liquid, suitable for plant cell culture

Synonym(s):

Murashige-Skoog medium

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NACRES:
NA.72
UNSPSC Code:
12352207

Key Documents

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Product Name

Murashige and Skoog Vitamin Solution, 1000 ×, liquid, suitable for plant cell culture

sterility

sterile-filtered

form

liquid

concentration

1000 ×

technique(s)

cell culture | plant: suitable

color

colorless

pH

5.5-7.5

application(s)

agriculture

shipped in

ambient

storage temp.

2-8°C

Quality Level

400

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This Item
M7150M0529G1019
Sigma-Aldrich

Sigma-Aldrich

M3900

Murashige and Skoog Vitamin Solution

Murashige and Skoog Vitamin Powder 1000 ×, powder, suitable for plant cell culture

Sigma-Aldrich

M7150

Murashige and Skoog Vitamin Powder

Sigma-Aldrich

Sigma-Aldrich

M0529

Murashige and Skoog Basal Salt Micronutrient Solution

Sigma-Aldrich

Sigma-Aldrich

G1019

Gamborg′s Vitamin Solution

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

form

liquid

form

powder

form

liquid

form

liquid

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

sterility

sterile-filtered

sterility

-

sterility

sterile-filtered

sterility

sterile-filtered

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Application

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).
Use at a concentration of one ml per liter of prepared medium to achieve the proper final concentration.

Preparation Note

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Vitamin powder (M3900) can be combined with Murashige and Skoog micro/macro-nutrients to generate basal media which when supplemented with organics, agar, auxins and cytokinins yield complete medium for growth plant tissue culture. The M3900 Solution is a 1000x concentrate which contains (mg/ml): 2.0 glycine, 100.0 myo-inositol, 0.50 nicotinic acid, 0.50 pyridoxine hydrochloride, 0.10 thiamine hydrochloride.
Solution contains (mg/ml): 2.0 glycine, 100.0 myo-inositol, 0.50 nicotinic acid, 0.50 pyridoxine hydrochloride, 0.10 thiamine hydrochloride.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
RNBL5855
RNBL0142
RNBK2254
RNBJ6958
RNBH9430

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Ting Li et al.
Nature communications, 10(1), 726-726 (2019-02-15)
In plants mechanical signals pattern morphogenesis through the polar transport of the hormone auxin and through regulation of interphase microtubule (MT) orientation. To date, the mechanisms by which such signals induce changes in cell polarity remain unknown. Through a combination
O Van Wuytswinkel et al.
The Plant journal : for cell and molecular biology, 17(1), 93-97 (1999-03-09)
Intracellular iron concentration requires tight control and is regulated both at the uptake and storage levels. Our knowledge of the role that the iron-storage protein ferritins play in plants is still very limited. Overexpression of this protein, either in the
Saxena et al.
Plant science : an international journal of experimental plant biology, 155(2), 133-140 (2000-05-18)
Efficient protocols have been established for both direct and indirect regeneration of plants in Pelargonium graveolens Indian cultivar Hemanti (Algerian type). Murashige and Skoog's (MS) medium [T. Murashige, F. Skoog, A revised medium for rapid growth and bioassays with tobacco

Questions

  1. do you have de product information ?

    1 answer

    1. The product detail page contains information such as properties, applications, components, and preparation instructions for this product. From the product detail page DOCUMENTATION section, additional information may be obtained, such as the Certificate of Analysis and SDS. Please see the documents below:

      SDS
      https://www.sigmaaldrich.com/sds/sigma/m3900

      COA
      https://www.sigmaaldrich.com/coa/SIGMA/M3900/RNBL5855

      For more information, we kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice and click on "Product Technical Inquires" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.

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