M4526

Minimum Essential Medium Eagle

Alpha Modification, with sodium bicarbonate, without L-glutamine, ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): αMEM, EMEM, MEM

Properties

• Quality Level: 400
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: Earle’s salts (5% CO₂): yes; sodium pyruvate: yes; glucose: yes; L-glutamine: no; HEPES: no; Hanks’ salts (2% CO₂): no; phenol red: yes; NaHCO₃: yes; stable glutamine: no
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has enhanced the uses of this medium. The α modification of MEM with Earle′s balanced salts also known as αMEM, contains non-essential amino acids, sodium pyruvate, and additional vitamins.

This is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins.

Application

Minimum Essential Medium Eagle has been used to maintain:

• immature cumulus-oocyte complexes from mice
• human mesenchymal stem cells (MSC)
• mouse pre-osteoblastic cell line

Reconstitution

Supplement with 0.292 g/L L-glutamine.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable