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M5650

Sigma-Aldrich

Minimum Essential Medium Eagle

With Earle′s salts, non-essential amino acids and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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Synonym(s):
EMEM, MEM
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: yes
NaHCO3: yes
L-glutamine: no
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes

shipped in

ambient

storage temp.

2-8°C

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This Item
M2279M0644M4526
Sigma-Aldrich

M5650

Minimum Essential Medium Eagle

Sigma-Aldrich

M2279

Minimum Essential Medium Eagle

Sigma-Aldrich

M0644

Minimum Essential Medium Eagle

Sigma-Aldrich

M4526

Minimum Essential Medium Eagle

form

liquid

form

liquid

form

powder

form

liquid

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

sterility

sterile-filtered

components

phenol red: yes
NaHCO3: yes
L-glutamine: no
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes

components

phenol red: yes
sodium pyruvate: no
L-glutamine: no
HEPES: no
NaHCO3: yes
Earle’s salts (5% CO2): yes

components

NaHCO3: no
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: yes

components

Earle’s salts (5% CO2): yes
sodium pyruvate: yes
glucose: yes
L-glutamine: no
HEPES: no
Hanks’ salts (2% CO2): no
phenol red: yes
NaHCO3: yes
stable glutamine: no

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has enhanced the uses of this medium. This formulation has been further modified by the optional elimination of calcium to permit the growth of cells in suspension culture. MEM can be used for the cultivation of a wide variety of cells grown in monolayers.

Application

Minimum Essential Medium Eagle has been used to culture:
  • HeLa cells
  • Madin-Darby canine kidney (MDCK) cells
  • chondrosarcoma cell line

This medium may be preferred for the growth of adherent cells in the presence of reduced serum or as a base for the development of a serum-free medium.

Reconstitution

Supplement with 0.292 g/L L-glutamine.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1


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Articles

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

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