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M7278

Sigma-Aldrich

Minimum Essential Medium Eagle

HEPES Modification, with Earle′s salts, 25 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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Synonym(s):
EMEM, MEM
NACRES:
NA.75

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

pH

>7.2

components

L-glutamine: no
phenol red: yes
HEPES: 25 mM
sodium pyruvate: no
Earle’s salts (5% CO2): yes
NaHCO3: yes

shipped in

ambient

storage temp.

2-8°C

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This Item
M0643M0450M4526
vibrant-m

M7278

Minimum Essential Medium Eagle

vibrant-m

M0643

Minimum Essential Medium Eagle

vibrant-m

M0450

Minimum Essential Medium Eagle

vibrant-m

M4526

Minimum Essential Medium Eagle

components

L-glutamine: no
phenol red: yes
HEPES: 25 mM
sodium pyruvate: no
Earle’s salts (5% CO2): yes
NaHCO3: yes

components

HEPES: no
Earle’s salts (5% CO2): yes
NaHCO3: no
phenol red: yes
L-glutamine: yes
sodium pyruvate: no

components

glucose: 1.0 g/L
L-glutamine: 0.292 g/L
phenol red: 0.011 g/L
NaHCO3: 2.2 g/L

components

Earle’s salts (5% CO2): yes
sodium pyruvate: yes
glucose: yes
L-glutamine: no
HEPES: no
Hanks’ salts (2% CO2): no
phenol red: yes
NaHCO3: yes
stable glutamine: no

sterility

sterile-filtered

sterility

-

sterility

sterile-filtered

sterility

sterile-filtered

form

liquid

form

powder

form

liquid

form

liquid

impurities

endotoxin, tested

impurities

-

impurities

Endotoxin, tested

impurities

endotoxin, tested

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

Minimum Essential Medium Eagle comprises sugar, inorganic salts, water-soluble vitamins and essential amino acids. It is widely used as a media formulation for many cell culture studies.

Application

Minimum Essential Medium Eagle has been used for culturing primordial follicles and ovary tissues, human skin fibroblast cells, human colonic epithelial cell line LS174T and hippocampal tissue slices.

Reconstitution

Supplement with 0.292 g/L L-glutamine.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Articles

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

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