MAK074

Ascorbic Acid Assay Kit

sufficient for 100 colorimetric or fluorometric tests

• Synonym(s): Vitamin C Assay Kit
• NACRES: NA.84

Properties

• usage: sufficient for 100 colorimetric or fluorometric tests
• detection method: colorimetric; fluorometric
• storage temp.: −20°C

Description

General description

Ascorbic Acid, also known as Vitamin C, is a six-carbon lactone produced by plants and some animal species but not by humans and other primates. Ascorbic acid functions as an enzymatic cofactor for multiple enzymes, serving as an electron donor for monooxygenases and dioxygenases. Ascorbic acid also functions as a powerful antioxidant, particularly in regards to reactive oxygen species.

Suitability

Suitable for use with biological fluids, food samples, and growth media. Proteins present in the sample can interfere with this assay. Protein containing samples are best analyzed using the Ascorbic Acid Assay II (MAK075). Alternatively, samples can be deproteinized using a 10 kDa Molecular Weight Cut-Off Spin Filter.

Principle

In this assay, Ascorbic Acid concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λ_ex = 535/ λ_em = 587 nm) product, proportional to the ascorbic acid present.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H317 - H319 - H334
• Precautionary Statements: P261 - P264 - P272 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): 188.6 °F - closed cup
• Flash Point(C): 87 °C - closed cup