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MBD0025

Sigma-Aldrich

Water

Microbial DNA-free; Suitable for PCR, sequencing and NGS

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Synonym(s):
DNA-free water, Filter-sterilized water, Microbial DNA-free water, Microbiome water, UV-treated water

Quality Level

form

liquid

feature

DNA free

technique(s)

DNA extraction: suitable
DNA sequencing: suitable
PCR: suitable

shipped in

ambient

storage temp.

room temp

Related Categories

General description

Microbial DNA-free water is filter-sterilized and UV- treated. It undergoes stringent quality control testing to ensure that it is free of any detectable levels of DNA. The quality tests guarantee the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.

Provided in 10 vials of 1.5 ml, enabling use of 1 tube per PCR setup to avoid contamination of your master stock.

Progression in Next Generation Sequencing (NGS) technology now allows identification and sequencing of microbes in relatively low quantities and in complex samples. Therefore, NGS is the method of choice for population identification in microbiome samples, as most of them include large variety of microbes and in some cases also host cells. Still, biases and errors are common in this workflow and can be introduced in different steps along this process. Reagents used must be free of any microbial contaminant to avoid introduction of exogenous error to the samples especially these with relatively low microbial counts

Application

Microbial DNA-free water is suited for use in PCR assays as a negative PCR template control (1). It has been used to dilute genomic DNA template in qPCR assay to detect and quantify microbial load in water and other environmental samples and for downstream applications, such as sanger sequencing and next-generation sequencing.(2, 3) It may be used in the preparation of PCR master mixes and reagents to prevent DNA contamination problems leading to false-positive signals in real-time polymerase chain reaction detection of bacterial pathogens in biological samples. (4)

Features and Benefits

DNA-free Water: Filter-sterilized and UV-treated to ensure the water is completely free of DNA microbial contaminant to avoid introduction of exogenous error

Highest quality: Strict quality control testing for absence of detectable levels of contaminating microbial DNA

Convenient: 1 tube per PCR setup for avoiding master stock contamination

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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