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N3643

Sigma-Aldrich

NZCYM Broth

Powder microbial growth medium

NACRES:
NA.85

grade

for molecular biology

Quality Level

sterility

non-sterile

form

powder

composition

Casamino Acids, 1 g/L
MgSO4, 15 g/L
NaCL, 5 g/L
Tryptone, 10 g/L
Yeast extract, 5 g/L

pH

6.8-7.2(2.2% solution)

application(s)

food and beverages

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

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This Item
N3518L3522L7658
NZCYM Broth Powder microbial growth medium

Sigma-Aldrich

N3643

NZCYM Broth

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N3518

NZ Amine® Broth

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L3522

LB Broth (Miller)

LB Broth (Lennox) EZMix™ powder microbial growth medium

Sigma-Aldrich

L7658

LB Broth (Lennox)

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

form

powder

form

powder

form

powder

form

powder

composition

Casamino Acids, 1 g/L , MgSO4, 15 g/L , NaCL, 5 g/L , Tryptone, 10 g/L , Yeast extract, 5 g/L

composition

MgSO4, 1 g/L , NaCl, 5 g/L , Tryptone, 10 g/L

composition

NaCl, 10 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L

composition

-

pH

6.8-7.2(2.2% solution)

pH

6.3-7.2(1.6% solution)

pH

6.8-7.2(2.5% solution)

pH

6.8-7.2(2.5% solution)

application(s)

food and beverages

application(s)

food and beverages

application(s)

food and beverages

application(s)

agriculture
food and beverages

General description

NZCYM is a microbial growth medium used for the cultivation of E. coli and lambda bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.

Application

NZCYM Broth has been used for non-selective cultivation of lambda, MS2, T7, CLP-P3, Phi29, and Goe1 bacteriophages, and E. coli strains for cloning, DNA plasmid production, and production of recombinant proteins. It is also suitable for selective cultivation when appropriate antibiotics are added.

Features and Benefits

NZCYM powder provides:
  • Standard formulation
  • Convenient package sizes
  • A budget-friendly alternative to liquid

Reconstitution

Stir to suspend 22 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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705578-5MG-PW

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MMYOMAG-74K-13

1000309185

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Protocols

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

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