All Photos(8)



Papain from papaya latex

lyophilized powder, ≥10 units/mg protein

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:

biological source

papaya (latex)

Quality Level


lyophilized powder

specific activity

≥10 units/mg protein

mol wt

23.4 kDa


diagnostic assay manufacturing

storage temp.


Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Papain is a cysteine protease, found in Carica papaya. It is composed of a single polypeptide chain containing three disulfide bridges and a sulfhydryl group.


Papain from papaya latex has been used:
  • to digest carotid artery smooth muscle cells
  • in dissecting solutions
  • to produce Fab fragments of antibodies
  • for cell dissociation since it has been shown to be more effective and less damaging with certain tissues
  • in the enzyme cocktail for dissociating human fetal retinal tissue
  • to digest chondrocytes/scaffold cultures for total DNA quantification for cell proliferation
  • as a supplement in neurobasal medium to produce retinal cell suspensions
Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.


25 mg in poly bottle
50, 100, 500 mg in glass bottle
1, 5 g in glass bottle
Package size based on protein content

Biochem/physiol Actions

Papain with wide specificity, cleaves peptide bonds of basic amino acids, leucine or glycine. It also hydrolyzes esters and amides. Papain is considered to digest protein substrates more broadly than the pancreatic proteases. Papain is commonly used in cell isolation procedures as it is highly efficient and less harmful than other proteases on certain tissues. The use of papain is known to yield more viable smooth muscle cells, retaining the cell′s sensitivity to stimulants. Papain papaya latex has antifungal activity against C. albicans and is a cysteine protease.
  • The pH optimum is 6.0-7.0
  • Papain hydrolyzes esters and amides
A cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
pH optimum 6.0-7.0
Also hydrolyzes esters and amides.

Unit Definition

One unit will hydrolyze 1.0 micromole of N-alpha-benzoyl-L-arginine ethyl ester (BAEE) per minute at pH 6.2 at 25 deg C.

Physical form

Lyophilized powder containing sodium acetate

Preparation Note

2× Crystallized

Other Notes

View more information on papain at


Exclamation markHealth hazard

Signal Word


Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Technical Information

More Documents


Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service