R1780

RPMI-1640 Medium

With L-glutamine and sodium bicarbonate. Without arginine, leucine, lysine, and phenol red, liquid, sterile-filtered, suitable for cell culture, designed for isotope labeling for cell culture applications

• MDL number: MFCD00217820
• NACRES: NA.75

Properties

• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: NaHCO₃: 2 g/L; phenol red: no; glucose: 2 g/L (Dextro); L-glutamine: 0.3 g/L
• shipped in: ambient

Description

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Reconstitution

Designed for use in stable isotope labeling with amino acids in cell culture applications.
Supplement with 0.100 g/L L-Arginine-¹³C₆, ¹⁵N₄ HCl (Product 608033), 0.100 g/L L-Leucine-¹³C₆, ¹⁵N (Product 608068), and 0.100 g/L L-Lysine-¹³C₆, ¹⁵N₂ (Product 608041).

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable