R7755

RPMI-1640 Medium

AutoMod^™, without L-glutamine and sodium bicarbonate, powder, suitable for cell culture

• Synonym(s): Roswell Park Memorial Institute 1640 medium
• MDL number: MFCD00217820

Properties

• Quality Level: 500
• form: powder
• technique(s): cell culture | mammalian: suitable
• components: sodium pyruvate: no; HEPES: no; NaHCO₃: no; L-glutamine: no; phenol red: yes
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Other Notes

Modified for autoclaving
Without L-glutamine and sodium bicarbonate.

Quantity

Formulated to contain 10.3 grams of powder per liter of medium.

Reconstitution

Supplement with 0.3 L-glutamine, 2.0 g/L sodium bicarbonate.

Legal Information

Auto-Mod is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable