Merck
All Photos(2)

RAB0244

Sigma-Aldrich

Human IL-10 ELISA Kit

Synonym(s):
IL-10, Interleukin-10
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type serum
sample type cell culture supernatant(s)
sample type plasma

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... IL10(3586)

General description

Interleukin-10 (IL-10) is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells and B cells.[1] Murine IL-10 is inactive on human cells.[2] The gene encoding this protein is localized on human chromosome 1q31-32.[3]

Immunogen

Recombinant Human IL-10

Application

For research use only. Not for use in diagnostic procedures.
Human IL-10 ELISA kit has been used for determining the levels of IL-10 in cell culture supernatant. [4][5]
Human IL-10 ELISA Kit has been used in enzyme-linked immunosorbent assay (ELISA) to detect interleukin-10 (IL-10) in the plasma of uremic patients undergoing hemodialysis (HD).[6]

Biochem/physiol Actions

IL-10 (interleukin 10) is an anti-inflammatory cytokine.[7] IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNF (tumor necrosis factor)-α.[8] Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions.[9] Polymorphisms in the IL-10 gene are associated with higher risk of preeclampsia, ankylosing spondylitis, nasopharyngeal carcinoma and basal cell carcinoma.[10][11][12][13] Polymorphism in IL-10 at -1082A/G is associated with diabetic nephropathy.[7]

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)

  • RABSTOP3ELISA Stop Solution (Item I)

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  • RABWASH420X Wash Buffer (Item B)

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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