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Stemline® II Hematopoietic Stem Cell Expansion Medium

Serum-free, contains L-glutamine, liquid, sterile-filtered, suitable for cell culture


Quality Level





storage condition

protect from light


cell culture | mammalian: suitable

shipped in

wet ice

storage temp.


General description

The second generation of Sigma′s hematopoietic stem cell expansion media family, Stemline II has been developed to optimize the balance of differientiated and undifferentiated cells while maximizing their expansion. Compatible with Hematopoietic Stem Cells (HSC) from bone marrow, cord blood, and mobilized peripheral blood, Stemline II has been shown to lead to significant increases in cell expansion from all three sources. Through flow cytometric analysis of clinical-scale expansions, Stemline II has also demonstrated a higher capacity than other commercially available media for the expansion of CD34+/CD38+ late progenitors required for short-term engraftment. Human cord blood cells expanded in Stemline Media demonstrate impressive self-revewal when transplanted into immunodeficient NOC/SCID mice, illustrating Stemline′s utility in true functional trial.

Stemline Hematopoietic Stem Cell Expansion Medium free of serum and all other animal-derived components with the exception of human serum albumin. This exclusion increases performance consistency and elimates safety risks associated with potential adventitious agents.

Produced in a GMP state-of-the-art facility, Stemline Hematopoietic Stem Cell Expansion Medium is clearly an excellent choice for your HSC applications.

Please click here to contact us if you are considering including the use of this product in an Investigational New Drug application.


Must be supplemented with growth factors and cytokines according to individual user protocols.

Legal Information

Stemline is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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