Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
SHC001
MISSION® pLKO.1-puro Empty Vector Control Plasmid DNA
Contains no shRNA insert
Synonym(s):
MISSION® Control Vectors
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product line
MISSION®
concentration
500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
shipped in
dry ice
storage temp.
−20°C
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This Item | SHC201 | SHC016 | SHC005 |
|---|---|---|---|
| concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA) | concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA) | concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA) | concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA) |
| product line MISSION® | product line MISSION® | product line MISSION® | product line MISSION® |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
General description
The MISSION pLKO.1-puro Control Vector is a lentivirus plasmid vector. The vector does not contain an shRNA insert and is useful as a negative control in experiments using the MISSION shRNA library clones.[1][2] This allows one to examine the effect of transfection on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The pLKO.1-puro Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
Application
MISSION® pLKO.1-puro Empty Vector Control Plasmid DNA has been used to synthesize and clone shRNAs (which target murine cluster of differentiation 36 (CD36), to generate lentiviral expression vectors.[3]
To see more application data, protocols, vector maps visit sigma.com/shrna.
Legal Information
Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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AMP-activated protein kinase (AMPK)-induced preconditioning in primary cortical neurons involves activation of MCL-1.
Anilkumar U
Journal of Neurochemistry, 124 (2013)
Cardiospecific CD36 suppression by lentivirus-mediated RNA interference prevents cardiac hypertrophy and systolic dysfunction in high-fat-diet induced obese mice.
Zhang Y
Cardiovascular Diabetology, 14 (2015)
Gregory J Baker et al.
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Articles
When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, continuing gene silencing.
Related Content
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What is the Department of Transportation shipping information for this product?
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Does Product SHC001, MISSION® pLKO.1-puro Control Vector, convey puromycin resistance to transfected cells?
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Yes, it has the puromycin resistance gene.
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Is Product SHC001, MISSION® pLKO.1-puro Control Vector, available as a glycerol stock?
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The control vectors are available in plasmid DNA or the lentiviral transduction format.
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Where is the hairpin inserted with Product SHC001, MISSION® pLKO.1-puro Control Vector?
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This control vector does not contain an shRNA insert.
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What is the function of this negative control with Product SHC001, MISSION® pLKO.1-puro Control Vector?
1 answer-
It permits observation of cellular effects resulting from the transfection process. Cells transfected with the empty control provide a useful reference point for determining specific knockdown.
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