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LIF human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

D factor, Differentiation-stimulating factor, Melanoma-derived LPL inhibitor (MLPLI)


expressed in E. coli


≥98% (HPLC)
≥98% (SDS-PAGE)



mol wt

19.6 kDa


endotoxin, tested

UniProt accession no.

shipped in

wet ice

storage temp.


Gene Information

human ... LIF(3976)

General description

Leukemia inhibitory factor (LIF) is a secreted pleiotropic glycoprotein which is a member of the gp130 signaling of interleukin-6 (IL-6) family cytokines. This protein was originally identified as a protein that prevented the growth of a leukemic cell line.
Recombinant human LIF is a 19.6 kDa protein containing 180 amino acids residues including three disulfide bonds.


25 μg

Biochem/physiol Actions

Leukemia inhibitory factor (LIF) suppresses the differentiation of myoblasts, and inhibits the activation of caspase-3 and blocks the reduction in Bcl-xL (B-cell lymphoma-extra large) levels, thus, preventing doxorubicin-induced apoptosis. Along with transforming growth factor β2 (TGFβ2)/fibroblast growth factor 2 (FGF2), LIF regulates nephrogenesis. It facilitates tubular regeneration following acute kidney failure. It plays a crucial role in neuroprotective pathways induced by preconditioning-induced oxidative stress. LIF is capable of maintaining the totipotency of embryonic stem cells. It is also involved in controlling the differentiation of embryonic stem cells, hematopoietic and neuronal cells.

Physical form

Lyophilized with no additives


Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

13 - Non Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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