SRP6287

LIF human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Synonym(s): CDF, DIA, HILDA, LIF, MLPLI
• NACRES: NA.32

Properties

• recombinant: expressed in HEK 293 cells
• assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• mol wt: calculated mol wt 19.9 kDa (with Gly-Pro at the N- terminus); observed mol wt 35-45 kDa (DTT-reduced.Ser 23 is the predicted N-terminus .)
• impurities: <1 EU/μg endotoxin (LAL test)
• UniProt accession no.: P15018
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... LIF(3976)

Description

General description

Leukemia inhibitory factor (LIF) is a secreted pleiotropic glycoprotein which is a member of the gp130 signaling of interleukin-6 (IL-6) family cytokines.[1][2] This protein was originally identified as a protein that prevented the growth of a leukemic cell line.[3]

Biochem/physiol Actions

Leukemia inhibitory factor (LIF) suppresses the differentiation of myoblasts, and inhibits the activation of caspase-3 and blocks the reduction in Bcl-xL (B-cell lymphoma-extra large) levels, thus, preventing doxorubicin-induced apoptosis. Along with transforming growth factor β2 (TGFβ2)/fibroblast growth factor 2 (FGF2), LIF regulates nephrogenesis. It facilitates tubular regeneration following acute kidney failure. It plays a crucial role in neuroprotective pathways induced by preconditioning-induced oxidative stress.[1] LIF is capable of maintaining the totipotency of embryonic stem cells.[3] It is also involved in controlling the differentiation of embryonic stem cells, hematopoietic and neuronal cells.[4]

Physical form

Sterile filtered through a 0.22 micron filter. Lyophilized from 1 x PBS, pH 7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable