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T9179

Sigma-Aldrich

Terrific Broth

EZMix® powder microbial growth medium

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Synonym(s):
TB media
NACRES:
NA.85

grade

for molecular biology

Quality Level

description

TB; quick dissolve, dust-free formulation

sterility

non-sterile

form

powder

technique(s)

microbiological culture: suitable

application(s)

agriculture
food and beverages
microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

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This Item
L35221.01629L2542
Terrific Broth EZMix™ powder microbial growth medium

Sigma-Aldrich

T9179

Terrific Broth

Sigma-Aldrich

Sigma-Aldrich

L3522

LB Broth (Miller)

Terrific broth suitable for microbiology

Millipore

1.01629

Terrific broth

LB Broth (Miller) Liquid microbial growth medium

Sigma-Aldrich

L2542

LB Broth (Miller)

sterility

non-sterile

sterility

non-sterile

sterility

-

sterility

sterile; 0.2 μm filtered

form

powder

form

powder

form

medium granules (dehydrated (DCM))

form

liquid

technique(s)

microbiological culture: suitable

technique(s)

-

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

application(s)

agriculture
food and beverages
microbiology

application(s)

food and beverages

application(s)

clinical
pharmaceutical, microbiology

application(s)

microbiology

storage temp.

room temp

storage temp.

room temp

storage temp.

15-25°C

storage temp.

room temp

General description

Terrific Broth is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains glycerol, peptides, amino acids, water-soluble vitamins, and carbohydrates.

Application

Suitable for non-selective cultivation of E. coli strains for cloning, high yield DNA plasmid production and high yield production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.
Terrific broth has been used:
  • as a medium for selective growth of E.coli BL21 (DE3) V2RpRARE expressing MLL SET (nuclear proto-oncogene) domain in pET28GST-LIC
  • to grow BL21 (DE3) cells containing plasmid expressing IL-8 (interleukin-8)
  • Terrific broth is also suitable for use in bacterial suspension (Bacillus subtilis strain 1085B)

Features and Benefits

Terrific Broth EZMix® powder provides:
  • Granulated, dust-free format for safer handling and faster mixing
  • A budget-friendly alternative to liquid
  • Modified formulation (without glycerol)
  • Easy scale-up using larger package sizes

Components

*Note some of the product sizes available are given in liquid measure. This is a powder format product and those sizes reflect the final reconstituted volume.

23.6g/L Yeast Extract
11.8g/L Tryptone
9.4g/L K2HPO4
2.2g/L KH2PO4

Reconstitution

Suspend 24.1 g of powder and 4 mL of glycerol in sufficient deionized water to make 500 mL. Autoclave for 15 minutes at 121 °C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Legal Information

EZMix is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Protocols

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

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