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Merck

W4502

Sigma-Aldrich

Water

suitable for electrophoresis, PCR

Water

Synonym(s):

DNase-Free Water, Not DEPC Treated, Nuclease-Free Water, Protease-Free Water, RNase-Free Water

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About This Item

Linear Formula:
H2O
CAS Number:
Molecular Weight:
18.02
Beilstein:
2050024
EC Number:
MDL number:
UNSPSC Code:
12191602
PubChem Substance ID:
NACRES:
NA.31
Technique(s):
PCR: suitable
electrophoresis: suitable
Bp:
100 °C (lit.)
Vapor pressure:
3 mmHg

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Product Name

Water, Nuclease-Free Water, for Molecular Biology

vapor density

<1 (vs air)

Quality Level

vapor pressure

3 mmHg

sterility

0.1 μm filtered

form

liquid

technique(s)

PCR: suitable
electrophoresis: suitable

refractive index

n20/D 1.34 (lit.)

pH

5-7

bp

100 °C (lit.)

mp

0 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

foreign activity

DNase, RNase, Protease, free

SMILES string

O

InChI

1S/H2O/h1H2

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

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1 of 4

This Item
9801-OP9528995284
technique(s)

PCR: suitable, electrophoresis: suitable

technique(s)

-

technique(s)

-

technique(s)

FISH: suitable

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

100

form

liquid

form

liquid

form

liquid

form

liquid

mp

0 °C (lit.)

mp

0 °C (lit.)

mp

0 °C (lit.)

mp

0 °C (lit.)

refractive index

n20/D 1.34 (lit.)

refractive index

n20/D 1.34 (lit.)

refractive index

n20/D 1.34 (lit.)

refractive index

n20/D 1.34 (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

Application

Water has been used-
  • in cDNA synthesis and PCR amplification[1][2]
  • for the dilution of rumen DNA sample prior to qPCR assay[3]
  • in PCR (polymerase chain reaction) and agarose gel electrophoresis[4]
  • for the elution of RNA during RNA isolation[5]

Features and Benefits

  • Nuclease-Free Water
  • DNase-Free, RNase-Free, Protease- Free
  • Not DEPC Treated
  • 0.1 um Filtered
  • Prepared from Double Distilled and Deionized Water
  • Suitable for use in Molecular Applications

This product is 0.1um filtered. It is designated as a Molecular Biology Reagent and is suitable for molecular biology applications. This product has been analyzed for the absence of nucleases and proteases, and has undergone bioburden analysis. It has not been treated with diethyl pyrocarbonate (DEPC).

Preparation Note

This product is prepared from double distilled and deionized water

Other Notes

Easily compare specifications for Water products with the Water specification table.

related product

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number

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Polymorphic exact tandem repeat A (PETRA): a newly defined lineage of mycobacterium tuberculosis in israel originating predominantly in Sub-Saharan Africa.
Freidlin PJ et al
Journal of Clinical Microbiology, 47(12), 4006-4020 (2009)
Effect of sward dry matter digestibility on methane production, ruminal fermentation, and microbial populations of zero-grazed beef cattle.
Hart KJ et al
Journal of Animal Science, 87(10), 3342-3350 (2009)
Presence of viral genomes in mineral water: a sufficient condition to assume infectious risk?
Gassilloud B et al
Applied and Environmental Microbiology, 69(7), 3965-3969 (2003)
Gene expression signals involved in ischemic injury, extracellular matrix composition and fibrosis defined by global mRNA profiling of the human left ventricular myocardium.
Gabrielsen A et al
Journal of Molecular and Cellular Cardiology, 42(4), 870-883 (2007)
Comparison of coliforms and coliphages as tools for assessment of viral contamination in river water.
Skraber S et al
Applied and Environmental Microbiology, 70(6), 3644-3649 (2004)

Protocols

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GenElute 哺乳动物基因组 DNA 纯化方案阐述了从多种哺乳动物来源中分离纯高分子量 DNA 的方法。

本方案描述了使用GenElute植物基因组DNA Miniprep试剂盒,从多种植物中分离纯DNA的简单、便捷操作步骤。

Imprint®甲基化DNA定量试剂盒提供了一种高通量、非放射性的整体DNA甲基化变化测定方法。

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GenomePlex®全基因组扩增是从动物样品中提取DNA的方法。GenomePlex®产品已用于扩增鸡、猪、牛、鱼和虾来源的基因组DNA。

Questions

1–5 of 5 Questions  
  1. W4502 Millipore Water: what is the ohms of this water?

    1 answer
    1. The conductivity of this water is not determined. Currently, there is no Molecular Biology grade water product available that is tested for conductivity.

      Helpful?

  2. Do you have stability data for W4502 for evaluating if Shelf Life can be extended?

    1 answer
    1. This water product is not assigned an expiration or retest date. In the original sealed container the material is stable nearly indefinitely. Items that are not assigned an expiration date are warranteed for 1 year from the date of shipment.

      Helpful?

  3. Is this water sterile? Can this be used for cell culture?

    1 answer
    1. Product W4502 is not sterile, this product is prepared from Double Distilled and Deionized Water. This product is best suitable to use in molecular biology applications. See product W3500 for Sterile, Tissue Culture grade Water:
      https://www.sigmaaldrich.com/product/sigma/w3500

      Helpful?

  4. In what vessel does this product come? A bottle, a bag with a spigot, etc.?

    1 answer
    1. This product is packaged in square PET square bottles. To request a quote for larger volumes or specialized packaging, please submit a request at the link below:
      https://www.sigmaaldrich.com/services/support/bulk-quotation-request

      Helpful?

  5. This product does not list an expiration date on the label. Could someone provide an expiration for lot number BCCH7145?

    1 answer
    1. This product does not contain an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services

      Helpful?

Reviews

Active Filters

  1. USA
    • Review 1
    • Votes 3
    1 out of 5 stars.

    This water has microbial contamination!

    We used this water (Lot #0000387784) to dilute DNA for cell culture, but the cells kept getting contaminated. Eventually we traced it back to the water. Even a freshly opened bottle had microbial growth after mixing with culture medium. This ended up ruining thousands of dollars of experiments.

    Helpful?

    1. Response from Merck KGaA:

      Thank you for your review. We are sorry to hear that your experience did not match your product expectations. We encourage customers who experience problems with our products to contact us for additional technical support. Please visit https://www.sigmaaldrich.com/US/en/support/customer-support to submit a Product Technical Inquiry.

  2. 1 Ratings-only review

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