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W4502

Sigma-Aldrich

Water

Nuclease-Free Water, for Molecular Biology

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Synonym(s):
DNase-Free Water, Not DEPC Treated, Nuclease-Free Water, Protease-Free Water, RNase-Free Water
Linear Formula:
H2O
CAS Number:
Molecular Weight:
18.02
Beilstein:
2050024
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.31

vapor density

<1 (vs air)

Quality Level

vapor pressure

3 mmHg

sterility

0.1 μm filtered

form

liquid

technique(s)

PCR: suitable
electrophoresis: suitable

refractive index

n20/D 1.34 (lit.)

pH

5-7

bp

100 °C (lit.)

mp

0 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

foreign activity

DNase, RNase, Protease, free

SMILES string

O

InChI

1S/H2O/h1H2

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

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1 of 4

This Item
W3500W175495289
Sigma-Aldrich

W4502

Water

Sigma-Aldrich

W3500

Water

Sigma-Aldrich

W1754

Water

Sigma-Aldrich

95289

Water

form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

PCR: suitable, electrophoresis: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

PCR: suitable

technique(s)

-

density

1.000 g/mL at 3.98 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

pH

5-7

pH

5-7

pH

5-7

pH

5-7

vapor pressure

3 mmHg

vapor pressure

3 mmHg

vapor pressure

3 mmHg

vapor pressure

-

Application

Water has been used-
  • in cDNA synthesis and PCR amplification
  • for the dilution of rumen DNA sample prior to qPCR assay
  • in PCR (polymerase chain reaction) and agarose gel electrophoresis
  • for the elution of RNA during RNA isolation

Features and Benefits

  • Nuclease-Free Water
  • DNase-Free, RNase-Free, Protease- Free
  • Not DEPC Treated
  • 0.1 um Filtered
  • Prepared from Double Distilled and Deionized Water
  • Suitable for use in Molecular Applications

This product is 0.1um filtered. It is designated as a Molecular Biology Reagent and is suitable for molecular biology applications. This product has been analyzed for the absence of nucleases and proteases, and has undergone bioburden analysis. It has not been treated with diethyl pyrocarbonate (DEPC).

Preparation Note

This product is prepared from double distilled and deionized water

Other Notes

Easily compare specifications for Water products with the Water specification table.

related product

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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T1503
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25G
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MMYOMAG-74K-13

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Polymorphic exact tandem repeat A (PETRA): a newly defined lineage of mycobacterium tuberculosis in israel originating predominantly in Sub-Saharan Africa.
Freidlin PJ et al
Journal of Clinical Microbiology, 47(12), 4006-4020 (2009)
Comparison of coliforms and coliphages as tools for assessment of viral contamination in river water.
Skraber S et al
Applied and Environmental Microbiology, 70(6), 3644-3649 (2004)
Gene expression signals involved in ischemic injury, extracellular matrix composition and fibrosis defined by global mRNA profiling of the human left ventricular myocardium.
Gabrielsen A et al
Journal of Molecular and Cellular Cardiology, 42(4), 870-883 (2007)
Presence of viral genomes in mineral water: a sufficient condition to assume infectious risk?
Gassilloud B et al
Applied and Environmental Microbiology, 69(7), 3965-3969 (2003)
Effect of sward dry matter digestibility on methane production, ruminal fermentation, and microbial populations of zero-grazed beef cattle.
Hart KJ et al
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