20278

Supelite^™ DAX-8

jar of 100 g

• Synonym(s): XAD-8

Properties

• form: solid
• Quality Level: 100
• packaging: jar of 100 g
• technique(s): HPLC: suitable; LPLC: suitable; ion chromatography: suitable
• matrix: acrylic ester
• matrix active group: polymer
• particle size: 40-60 mesh
• pore size: ~0.79 mL/g pore volume; 225 Å mean pore size
• surface area: 140 m²/g
• density: 1.09 g/mL at 25 °C (true wet)(lit.); 1.23 g/mL at 25 °C (skeletal)(lit.)
• separation technique: reversed phase

Description

General description

Supelite^™ DAX-8 is a nonfunctionalised resin with strong hydrophobic organic matter endorsed to humic and fulvic acids. It is also referred as polymethylmethacrylate resin.

Application

Supelite^™ DAX-8 was used as an absorbent in:

• HPLC-SEC fractionation system
• column chromatography
• extraction procedure and extracts were analysed using Cary Eclipse fluorescence spectrophotometer

Adsorbent resin with moderate polarity for compounds up to 150,000 MW. Most commonly used for adsorption of fulvic and humic acids, treatment of paper pulp mill waste, and removal of alcohols, surfactants, and colorants from samples.

Preparation Note

DAX-8 is a salt imbibed resin, which requires some preparation prior to use. In the protocol below, you may use methanol in place of ethanol.

• First, rinse the adsorbent resin with 2-3 bed volumes (BV) of distilled water.
• Soak overnight in 2 BV of 95% ethanol.
• After soaking, the resin is introduced into the column and rinsed with an additional 2 BV of 95% ethanol.
• Rinse with 2 BV of distilled water to displace the ethanol, followed by 1 BV of 4% (w/v) sodium hydroxide, 2 BV of distilled water, 1 BV 4% (v/v) hydrochloric acid, and finally by distilled water until the pH of the eluent becomes neutral.

Legal Information

Supelite is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves