Chelating & Reducing Agents for Research
We offer a wide range of chelating and reducing agents in high-purity grades and diverse forms for biochemical research.
Chelators, or chelating agents, are found in biological substances and are utilized for the removal of heavy metal impurities, which can be often toxic, and restore cellular metabolism. Through their ring structures, chelators can establish two or more bonds with metal ions, resulting in the formation of water-soluble, stable complexes. Ethylenediamine, EDTA, and EGTA are well-known examples of chelators.
Conducting both protein biochemistry analysis and oxidation-reduction reactions necessitates the use of reducers or reducing agents, also known as reductants. Reductants donate electrons in a redox chemical reaction. Several common reducing agents are DL-Dithiothreitol (DTT), sodium dithionate, DTE, TCEP, β-mercaptoethanol (BME), and Nitrilotriacetic acid (NTA).
Products
EGTA, Molecular Biology Grade
β-Mercaptoethanol, Molecular Biology Grade
A disulfide-reducing agent.
EDTA, 0.5 M, pH 8.0, Molecular Biology Grade, DEPC-Treated
Sterile-filtered solution of 0.5 M EDTA in H2O treated with diethyl pyrocarbonate (DEPC). Suitable for use in molecular biology applications.
DTT
OmniPur® Grade, >= 99.4%, Used for reduction of protein disulfide linkages
EDTA, Disodium Salt, Dihydrate, Molecular Biology Grade
EDTA
ULTROL® Grade, 500 mM Solution, pH 8.0
Deferoxamine Mesylate
Iron chelating agent
TCEP, Hydrochloride
A water-soluble and odorless disulfide-reducing agent that is more stable and effective than DTT.
1,4-Dithiothreitol
for biochemistry
Ethylenediaminetetraacetic acid disodium salt dihydrate
OmniPur® Grade, >= 99.0%
0.5M EDTA
Sputolysin® Reagent
Intended for the isolation of epithelial cells, pathogenic or saprophytic bacteria, fungi, and yeasts from sputum.
TCEP, Neutral
A ready-to-use stable, neutral form of the popular disulfide-reducing agent TCEP, Hydrochloride.
TPEN
Cleland′s Reagent
A protective agent for SH groups. Cyclizes as it reduces disulfides to thiols, so reaction is "driven" to completion. Does not contain waxy surfactant found in many other preparations. Oxidized dithiothreitol: ≤0.5%.
EGTA/AM
Membrane-permeable form of the Ca2+-chelating agent EGTA.
TCEP, Hydrochloride, Reagent Grade
BAPTA/AM
Membrane-permeable form of BAPTA.
THP
A water-soluble, neutral, and odorless disulfide reducing agent that is more stable and effective than DTT.
BAPTA, Tetrasodium Salt
Ca2+ chelator exhibiting a 105-fold greater affinity for Ca2+ .
CHELATING AGENTS IN RESEARCH
Chelators are used as complexing agents. They are helpful in various chemical and biochemical analyses as a buffer component, nucleic acid/protein degradation inhibitor, enzymatic reaction catalyzer, affinity chromatography, and cell culture. Chelators are potent therapeutic agents to balance metabolism and associated diseases such as anemia, cancer, acute kidney disease, aging, neurodegenerative disorders, etc. In industrial, analytical, and laboratory experiments, these metal-ion chelators are used as extractants, indicators, and metal separation agents.
REDUCING AGENTS IN RESEARCH
Reducers are utilized in proteomics methods such as protein purification, denaturation, and solubilization and are particularly adept in stabilizing free sulfhydryl groups and reducing disulfide (SH) bonds in proteins and peptides. In protein purification, they act as enzyme stabilizers within buffers, preventing aggregation by cysteine residue oxidation. Reducers also serve as biological antioxidants, averting protein oxidation and denaturing ribonucleases. Research has demonstrated that using chelating reagents in combination with reducing agents supports the removal of cationic heavy metals and anionic contaminants, such as arsenic.
Related Resources
- Article: Chelators
BioUltra Chelators. A chelate is a water-soluble complex between a metal ion and a complexing agent. It usually does not dissociate easily in solution, but forms an inert complex.
- Protocol: Nuclear Protein Extraction Without the Use of Detergent
Detergent-free procedure is recommended for nuclear protein preparation to avoid interference with labeling efficiency of extracted proteins.
- Calculators & Apps: Detergent-based Western Blot Stripping Buffer
This recipe for a detergent-based stripping solution enables the removal of antibodies from Western blots that have been developed with chemiluminescence or radioactive iodine or other isotopes.
- Flyer: Biochemicals for New and Growing Labs
Discover how our high-purity biological buffers, detergents, and reagents can help empower your innovation and build a better lab.
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