only
05 651 522 001
Cellavista Medium
Magnification: 4×, 10×,
20×
Illumination: Brightfield
and Fluorescence, UV,
Blue, Green
05 651 549 001
Cellavista High End
Magnification: 2×, 4×,
10×
Laemmli, U.K., Nature 227:680 (1970)
2. Weber, K . and Osborn, M., J. Biol. Chem.
244:4406 (1969)
3. Davies, G. and Stark, G., Proc. Nat. Acad. Sci.
USA, 66:651 (1970)
8/00
retains activity for at least two
years.
References
1. Narahashi, Y., Methods Enzymol., 19, 651-654
(1970).
2. Rebeyrotte, P., and Labbe, J.-P., C. R. Acad. Sc.
Paris, 274D, 2370-2373 (1972).
3. Vosbeck
al., Agr. Biol. Chem., 44(8),
1987-1988 (1980).
6. Maekawa, E. et al., Eur. J. Biochem., 200(3),
651-661 (1991).
The life science business of Merck operates
Bioi. Chem. 244:4406 (1969)
3. Davies, G., Stark, G., Proc. Nat. Acad. Sci. USA, 66:651 (1970)
@1988 Sigma Chemical Company
C HEM IC A I CQ ~'A'.'.A'k'~
~ ~ ':if';"7'~~'!11;~\
P.O. ~X 14508,$T
Bioi. Chem. 244:4406 (1969)
3. Davies, G., Stark, G., Proc. Nat. Acad. Sci. USA, 66:651 (1970)
@1988 Sigma Chemical Company
C HEM IC A I CQ ~'A'.'.A'k'~
~ ~ ':if';"7'~~'!11;~\
P.O. ~X 14508,$T
Darnell, J.E., STATs: transcriptional
control and biological impact., Nat. Rev. Mol. Cell.
Biol., 3, 651-662 (2002).
6. Stephens, J.M., et al., Activation of Signal
Transducers and Activators of Transcription
) Reprogramming of human primary somatic cells by Oct4 and chemical
compounds. Cell Stem Cells 7: 651-655.
10. Li Y, et al. (2010) Generation of iPSCs from mouse fibroblasts with a single gene, Oct4
Reprogramming of human primary somatic cells by Oct4 and chemical
compounds. Cell Stem Cells 7: 651-655.
10. Li Y, et al. (2010) Generation of iPSCs from mouse fibroblasts with a single gene, Oct4 and
efficient endogenous human gene
correction using designed zinc-finger nucleases.
Nature 435, 646-651 (2005).
For the most up to date reference list, please visit our website
at sigma-aldrich.com/life-science
efficient endogenous human gene
correction using designed zinc-finger nucleases.
Nature 435, 646-651 (2005).
For the most up to date reference list, please visit our website
at sigma-aldrich.com/life-science
efficient endogenous human gene
correction using designed zinc-finger nucleases. Nature 435,
646-651 (2005).
For the most up to date reference list, please visit our website at
http://www.sigmaaldrich.com
J.E. Darnell, STATs: transcriptional
control and biological impact. Nat. Rev. Mol. Cell.
Biol., 3, 651-662 (2002).
6. Stephens, J.M., et al., Activation of Signal
Transducers and Activators of Transcription
will lead to high cell death.
7. Centrifuge at 200 rpm for 5 minutes.
8. Remove the supernatant and resuspend the cell pellet in 2 mL NIM 2. Set aside.
9.
Bestimmung der
Kollagenase. Z. Physiol. Chem. 333, 149.
2 Matsubara, H. (1970) Purification and assay of thermolysin. Methods in
Enzymology 19, 642-651.
Modifying
Factors
3 Bond, M.D. & Van Wart, H.E
plate with 2 ml of medium in each well and prewarm in a CO2 incubator at 37 ºC for at least
20 minutes prior to nucleofection.
vi. Centrifuge 2 × 106 cells per transfection (8 × 106 total) at
R5145 6195 RPS6KA1 P NP_001006666.1 y y y
654 S6 Kinase S4047 6198 RPS6KB1 P NP_003152.1 y y y
651 S-100 (a-Subunit) S2407 6271 S100A1 M NP_006262.1 y n/d n/d
652 S-100 (?-Subunit) S2532 6285 S100B
R5145 6195 RPS6KA1 P NP_001006666.1 y y y
654 S6 Kinase S4047 6198 RPS6KB1 P NP_003152.1 y y y
651 S-100 (a-Subunit) S2407 6271 S100A1 M NP_006262.1 y n/d n/d
652 S-100 (?-Subunit) S2532 6285 S100B