Merck
Search Within
Applications
Content Type
Applied Filters:
Applications:Cytology and microscopy
Content Type:Protocol
Showing 1-9 of 9 results
Sort by Relevance
Enzymatic Method for Determining Urea (Phenylalanine Assay)
Assay protocol for the colorimetric detection of urea in biological samples using the urea assay kit.
How to Make and Use Percoll Gradients
To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.
Cell Dissociation Protocol using Trypsin
Trypsin is commonly used for dissociating adherent cells from surfaces. A wide variety of trypsin solutions are available to meet your specific cell line requirements.
Enzymatic Hydroxyproline Assay Protocol
Assay protocol for the colorimetric detection of Hydroxyproline in biological samples using the Hydroxypoline assay kit.
Suitability for the qualitative determination of calcium with phthalein purple (O-cresophthalein complexone) using stannous chloride
Suitability for the Qualitative Determination of Calcium with Phthalein Purple (O-Cresophthalein Complexone) using Stannous Chloride
Nuclear Protein Extraction Without the Use of Detergent
A protocol for the preparation of nuclear proteins without the use of a detergent. Detergents can interfer with the labeling efficiency of the extracted proteins.
Subculture of Adherent Cell Lines
Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.
Thawing of Frozen Cell Lines
Cell culture protocol for proper thawing of cryopreserved cell lines.
Nuclear Protein Extraction Without the Use of Detergent
Detergents can interfere with the labeling efficiency of the extracted proteins. Therefore, a procedure that does not include the use of detergents should be used for the preparation of nuclear proteins.