Cytology and microscopy

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Applications of Quantum Dots in Bioimaging and Bioassays

Professor Xiaohu Gao (University of Washington, USA) provides a overview of recent quantum dot (QD) advancements and their potential for advancing bioassay and bioimaging technologies.

Identification of Microorganisms Based on Color

For microbiologists the most fundamental stain was developed in 1884 by the Danish bacteriologist Hans Christian Gram.

Water for Histology Staining

Reliable histology staining requires consistent quality pure water. See examples of GMS and H&E stains performed with various water contaminants and comparing tap vs Type 2 water.

Primary Cell Culture Basics

Overview of primary cells and media usage in biomedical culture applications highlights differences from cell lines.

Cell Migration & Invasion Assays

Study tumor metastasis via various migration and invasion assays like Boyden chamber assay, Millicell® µ-Migration Assay, and scratch assay.

Apoptosis Assays

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Centrifugation Separations

CsCl gradient centrifugation separates RNA from DNA; differential and density gradient centrifugation techniques explained.

Evolution of Cell Culture Surfaces

For good cell attachment the hydrophobic polystyrene surface must be modified to a more hydrophilic surface. This allows cell attachment proteins (vitronectin and fibronectin) found in the serum containing culture medium to adhere and spread on the vessel bottom providing