Gene expression and silencing

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Technical bulletins for our shRNA products, including Bacterial Glycerol Stock, Purified Plasmid DNA Format, Lentiviral Format, MISSION® Control Vectors, MISSION Control Transduction Particles, MISSION Lentiviral Packaging Mix.

siRNA Design, Manufacturing, Transfection & Detection

RNA interference (RNAi) is a natural biological mechanism wherein short inhibitory RNA (siRNA) duplexes induce potent inhibition of gene expression.

Lentiviral Transduction

In this exercise we will be demonstrating lentiviral transduction on two different cell lines.

TRC Library Citations

TRC 1.5 human and mouse shRNA libraries

MISSION® Application Data

MISSION® Application Data. We are committed to providing you with helpful application notes and data for the MISSION® TRC shRNA libraries.

Delivery of Nucleic Acids Using Polymers

This review will focus on the sub-sector of drug delivery concerning the delivery of nucleic acids, with a particular focus on poly(ethyleneimine).

CRISPR-based Gene Activation

Sigma has developed a gene activation system based on a fusion of dCas9 to the catalytic histone acetyltransferase (HAT) core domain of the human E1A-associated protein p300.

Successful Transduction Using Lentivirus

Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.

Phosphorothioate Oligonucleotides

Phosphorothioate is a widely used backbone variant effective in creating oligonucleotides for antisense knockdowns.

Oligonucleotide Quality Control & Quality Assurance

The foundation of Sigma's manufacturing process is a robust QMS, which drives compliance to ISO 9001:2008 certification.

siRNA Delivery in Mammalian Cell Lines

siRNAs are effectively delivered into mammalian cell lines that have been difficult or even impossible to transfect with siRNA when using other commercially available lipofection reagents.

shRNA Process and Diagram

When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, continuing gene silencing.

Experimental Design and Analysis — Experimental Design FAQs

We answer your frequently asked questions about designing shRNA experiments, including appropriate controls, screening techniques, selection and more.

esiRNA FAQs (Frequently Asked Questions)

esiRNA are endoribonuclease prepared siRNAs that target the same mRNA sequence for gene silencing. Here are some of the most asked questions regarding esiRNA uses and availability.

esiRNA Knockdown Efficiency Tested by Western Blotting

Quantitative and qualitative western blotting to validate knockdown by esiRNA.

MISSION^® Lentiviral shRNA

MISSION shRNA reduce the expression of specific target genes by targeting the specific mRNA therefore reducing the corresponding protein expression.

X-tremeGENE™ siRNA Transfection Reagent

Small inhibitory RNAs offer easy gene expression knockdown in mammalian cells, revolutionizing gene research.

Bioluminescent Firefly Luciferase Assays

Firefly luciferase is a sensitive reporter for gene studies due to its absence in mammalian cells or tissues.

miRNA (microRNA) Introduction

Mature microRNAs regulate gene expression through various mechanisms; partially complementary to mRNA molecules, downregulating gene expression.

Introduction to Cell Transfection

Transfection introduces genetic material into cells, aiding research in gene expression and cell biology.

Examination of Lentiviral Library Efficiency

Small interfering RNAs (siRNAs) are powerful tools for gene expression knockdown, widely used in molecular biology.

Probe QPCR for Gene-Level Expression

qPCR investigates gene expression, amplification, and alterations, crucial for tumor biology and understanding cancer genetics.

Next-Gen RNA Interference with MISSION^® shRNA

RNAi technology advancements enable gene expression silencing through endogenous RNAi pathways, applicable across eukaryotes.

DNA Oligonucleotide Synthesis

Learn about the steps in phosphoramidite solid-phase method of Oligonucleotide Synthesis.

MISSION^® shRNA Lentiviral Transduction

Lentiviruses can deliver significant amounts of genetic information into host cells. The resulting lentiviral particles were used to infect the human lung cancer cell line, H1299.

Oligonucleotide Quality Control by Mass Spectrometry

Mass Spectrometry is the technology of choice for analyzing oligonucleotide synthesis. It enables the most sensitive detection of low levels of by-products, which can affect performance.

Lentivirus Cell Line

Using lentivirus as a means to deliver shRNAs has become standard practice in many labs exploring RNAi.

Using Microarray Technology to Select Housekeeping Genes in Chinese Hamster Ovary Cells

In the present study, we have identifi ed species-specifi c housekeeping genes (HKGs) for Chinese Hamster Ovary (CHO) cells using data from microarray gene expression profiling.

Analysis of Fluorescent Dyes Used for Labeling DNA in Microarray Experiments

An article concerning Analyzing Properties of Fluorescent Dyes for Labeling DNA in Microarray Experiments presented by Sigma-Aldrich.com

Reverse Transfection of Plasmid DNA

Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.

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