Merck
Search Within
Applications
Content Type
Collections
Applied Filters:
Applications:Mammalian cell culture
Content Type:Technical Article
Showing 1-30 of 127 results
Sort by Relevance
Platelet Derived Growth Factors (PDGF)
Platelet-Derived Growth Factor is the principal mitogen found in mammalian serum and is released from platelets during clot formation.
Cell Culture Protocol 10: Testing Cells for Mycoplasma Contamination by Hoechst DNA Staining
Cell culture protocol for testing cell lines for mycoplasma contamination using indirect DNA staining using Hoechst 33342. Free ECACC handbook download.
The Cell Culture Laboratory and Equipment Overview
Watch a video about the Cell Culture Laboratory and Equipment Overview
Application of Corning® CellSTACK®-5 Chamber with the Corning CellBIND® Surface to Simplify and Enhance Mass-Production of Recombinant Proteins
Discover how the Corning CELLSTACK-5 Chamber is used to simply and enhance the mass production of recombinant proteins.
Fibronectin
Fibronectin (FN) is a multifunctional, extracellular plasma glycoprotein produced by hepatocytes that circulates at near micromolar concentration and assembles into extracellular matrix fibrils at cell surfaces along with locally produced cellular FN.
Counting of Cells
Trypan blue method of cell counting is inexpensive and readily available method. The method is based on principle that living cells exclude trypan blue dye and appear bright, while dead cells take up dye and appear blue. Watch video to
Other Classic Media
A large collection of more specialized media for you cell culture applications, including L-15 Medium (Leibovitz), Fischer's Medium, Iscove's Modified Dulbecco's Medium (IMDM) and many more
Iscove's Modified Dulbecco's Media (IMDM) Formulation
Iscove's Modified Dulbecco's Media (IMDM) Formulation
Cell Culture Fundamentals: Sourcing of Cell Lines
The European Collection of Authenticated Cell Cultures (ECACC) is one of the world’s largest biological cell bank sources supplying a diverse range of authenticated cell lines all offered through Sigma Aldrich. Free ECACC handbook download.
Medium 199 - M199 Formulations
When properly supplemented, Medium 199 (M199) has broad species applicability, particularly for cultivation of non-transformed cells. It is widely used in virology, vaccine production and in vitro cultivation of primary explants of mouse pancreatic epithelial and rat lens tissues.
Nutrient Mixture F-12 Ham Formulation
Nutrient Mixture F-12 Ham Formulation
McCoy's 5A Modified Media Formulation
McCoy's 5A Modified Media Formulation
F-12 Coon's Modification Formulation
F-12 Coon's Modification Formulation
Williams' Medium E Formulation
Williams' Medium E Formulation
Alternative High-Yield Cell Culture Systems
Solutions for high-yield cell culture scale-up applications including multi-layered flasks (HyperFlasks/CellStacks), roller bottles, spinner and shaker flasks. Free ECACC handbook download.
Good Cell Banking Practices
Overview of good cell banking practices for cell line cryopreservation purposes.
Hepatocyte Growth Factor (HGF)
Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of other cells, including endothelial and epithelial cells, melanocytes, and keratinocytes.
Quality Control Considerations in Cell Culture
Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.
Cysteine in Cell Culture
Importance and uses of cysteine in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures
Media Formulations
Compare all of the formulation tables for cell culture in one place.
Cell Culture FAQs: Bacterial Endotoxin Contamination
What is endotoxin? Frequently asked questions about bacterial endotoxin contamination of in vitro cell cultures. Details about how to endotoxin test using the LAL assay, common sources of laboratory endotoxin contamination and tips on how to avoid endotoxin contamination when
Serum for Cell and Tissue Culture FAQs
Find answers to frequently asked questions (FAQs) about FBS and other serum products, and links to related products.
3D Neurosphere Culture Media
3D neurosphere culture protocol to expand human U-87 MG glioblastoma cells in serum-free/xeno-free stem cell culture media. Explore over 350 PromoCell products on Sigma.com.
DMEM/nutrient Mixture F-12 Formulation
Dulbecco's Modified Eagle's Medium/Ham's Nutrient Mixture F-12 (DME/F12) Formulation
Dulbecco's Modified Eagle's Medium (DME) Formulation
Dulbecco's Modified Eagle's Medium (DME) Formulation
Folic Acid Metabolism in Cancer
This issue of Biofiles reviews some of our newest and most innovative technologies and their specific applications toward cancer research. In preparing this issue of Biofiles, one is reminded how complex the disease of cancer is, and how difficult it
Poor Adherent Cell Attachment Troubleshooting
Cells that are cultured under suboptimal growth conditions attach poorly. When the culture surface provided is appropriate, the most common cause of failure of cells to attach to a substrate is environmental stress placed on the cells.
Cell Culture Contamination Troubleshooting
Common Cell Culture Problems: Contamination is easily the most common problem encountered in cell culture laboratories, sometimes with very serious consequence.
Common Cell Culture Problems: Precipitates
Common Cell Culture Problems: Precipitates -- When contamination is ruled out, turbidity in cell culture media is often explained by the precipitation of metals, proteins, and other media components. Precipitates can be harmful to cell health, as they may alter
Extracellular Matrix Proteins and Tools for Cell Culture Optimization
Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.
Page 1 of 5
Page 1 of 5