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Integration of Sigma® TransPlex® WTA and the Complete WTA2 Kits with the NimbleGen™ Expression Microarray Workflow
Amplification products generated by the TransPlex® WTA and Complete WTA2 kits are suitable for microarray target for expression analyses, and can be readily integrated into existing NimbleGen workflows.
Inhibitor Resistant Genotyping PCR ReadyMix™
Inhibitor Resistant Genotyping PCR Readymix is a 2X concentrated ready-to-use reaction cocktail for PCR amplification of DNA templates that overcomes inhibitors often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues.
Protocol for DNA extraction & WGA Amplification from Soil Samples
Genomic DNA from soil samples can be easily damaged by nucleases and contaminating debris resulting in low DNA yields. As a result, the researcher’s ability to perform downstream analysis may be compromised. After isolating DNA from the soil sample, the
FFPE Tissue
Archived Formalin-fixed, Paraffin-embedded (FFPE) tissue samples are invaluable resources for profiling gene expression and studying a variety of diseases.
Buccal DNA Extraction & WGA Amplification Protocol
This protocol provides a simple and convenient method to isolate, amplify, and purify genomic DNA from buccal swabs. Buccal swabs are a convenient method of acquiring a DNA sample.
Plant-Based PCR Inhibitors During DNA Extraction and PCR Master Mix Performance
Evaluating the effects of PCR inhibitors and PCR enzyme selection using plant genomic DNA, GenElute™-E Single Spin Plant Extraction Kit, KOD-One™ PCR Master Mix and competitor high fidelity PCR master mixes.
Amplification of Genomic DNA using REDTaq® DNA Polymerase
Method for PCR using genomic DNA or other complex templates
Complete Whole Transcriptome Amplification Kit Protocol (WTA2)
WTA2, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3-bias
Reverse Transcription Protocol
Method for reverse transcription of RNA into DNA. Uses a premixed reagent that contains reverse transcriptase, dNTPs, primers, RNase inhibitor and buffer. Fast generation of cDNA.
Standard Reverse Transcription Protocol (Two-step)
Reverse transcription is the analysis of gene expression to measure concentration mrna a gene. there are several challenges such analyses, as differences in halflife between different transcripts, temporal patterns and lack correlation protein.
Animal Tissue DNA-Extraction & WGA Amplification Protocol
GenomePlex® Whole Genome Amplification is the method of extracting DNA from the animal sample. GenomePlex® products have been used to amplify genomic DNA from chicken, porcine, bovine, fish, and shrimp source.
Standard PCR Protocol
Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.
Whole Blood
Whole blood is a common source of material used to perform genetic analysis. Many times genomic DNA isolated from whole blood samples is of low yield.
Hot Start dNTP protocol to reduce non-specific amplification
Protocol using hot start dNTPs. Method includes modified nucleoside triphosphates that block DNA polymerase nucleotide incorporation during hot start PCR to increase specificity. Compatible with a variety of PCR reagents.
Circular DNA Amplification using TempliPhi™
This page shows circular DNA amplification using TempliPhi™ from Cytiva.
GenomePlex® Single Cell Whole Genome Amplification Kit (WGA4) - Protocol
GenomePlex® is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative amplification of genomic DNA
Amplification of Damaged DNA with Restorase DNA Polymerase (R1028)
Method for amplification of DNA from damaged DNA sources. Particularly useful for DNA extracted from old samples.
Genomic DNA Extraction and Amplification Procedure using REDExtract-N-Amp™ Tissue PCR Kit
The REDExtract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
Therma™ PCR Additive
PCR additive for the annealing and extension steps that reduce scatter and increase product signals.
SEQR - SeqPlex RNA Amplification Kit Protocol
The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.
Successful Amplification of Thermally Degraded DNA Using PCRboost™
DNA samples are often exposed to harsh environmental conditions, including heat, humidity, UV or chemicals. Some procedures, such as the extraction of samples from formalin-fixed paraffin embedded tissue samples, subject DNA to multiple extreme conditions.
Antibody-Enzyme Mediated Hot Start PCR Protocol
JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.
Reverse Transcription Protocol Using SYBR Green Dye Detection
Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.
Digital PCR
Digital PCR is an end-point PCR method that is used for absolute quantification and for analysis of minority sequences against a background of similar majority sequences, e.g., quantification of somatic mutations.
Titan™ One Tube RT-PCR System Protocol & Troubleshooting
Titan One Tube RT-PCR System Protocol & Troubleshooting
Low Contaminant Amplification of DNA Using MTP™ Taq DNA Polymerase
Method for bacterial genome analysis and detection of pathogens. Minimize false positive PCRs through lab design and reagents tested for use in bacterial PCR applications.
REDExtract-N-Amp™ Tissue PCR Kit Protocol
The exrract-N-Amp kit protocol provides a rapid DNA extraction method that leads to a PCR-ready sample in 15 minutes. Optimized PCR ReadyMix yields successful and clean amplification. RED loading dye allows for sample tracking.
Kapa Biosystems Taq DNA Polymerase and PCR Kits
Robust Taq DNA polymerase reagents and PCR kits for efficient extraction, purification, and amplification of routine and challenging DNA template sequences.
Long and Accurate PCR Amplification of DNA
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.
Rapid Genotyping of Mouse Tissue Using Extract-N-Amp Tissue PCR Kit
Genomic detection of DNA via PCR amplification and detection on an electrophoretic gel is a standard way that the genotype of a tissue sample is determined. Conventional preparation of tissues for PCR-ready DNA often take several hours to days, depending
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